Inhibitory Effects and Control Efficacy of Paenibacillus polymyxa Strain L1-9 on Alternaria solani of Tomato
MA Gui-zhen1，2, WANG Shu-fang1, BAO Zeng-hai1，2, WU Shao-jie1, XIA Zhen-qiang1, LI Shi-dong3
1Key Laboratory of Marine Biotechnology in Jiangsu Province, Huaihai Institute of Technology, Lianyungang 222005, Jiangsu, China; 2Jiangsu Institute of Marine Resources Development, Lianyungang 222005, Jiangsu, China; 3Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Abstract：L1-9 is a Paenibacillus polymyxa strain isolated from sediment of Lianyungang harbor. In this paper, the antimicrobial activities of L1-9 strain and its fermentations and culture filtrate against Alternaria solani of tomato（Lycopersicon esculentum Mill.）were detected through dual culture methods on PDA medium and agar-well diffusion method. The results indicated that fermentations and culture filtrate of L1-9 strain had obvious antimicrobial effects on restraining conidiophores sprouting of Alternaria solani strain. The inhibition zones got 17.6 mm and 15.8 mm, respectively, after 4 days culture. Fermentations and culture filtrate of L1-9 strain could restrain germ tube elongation of Alternaria solani strain. Moreover, fermentations had stronger antimicrobial effects than its culture filtrate. Biological control functions in laboratory were tested by 2 screening methods: detached leaf method and pot method. The results showed that fermentations of L1-9 and its culture filtrate had better control efficacies against tomato early blight. And the control efficacies of fermentations are better than culture filtrate. The protective and therapeutic effects got 79.28 % and 67.20 %, respectively.
