Abstract：Glucosinolates is a group of important secondary metabolites found in cruciferous plants. They and their degradation products have important biological properties. In recent years, with genome sequencing and functional analysis in model plant Arabidopsis thaliana, people understand more about metabolic pathways of glucosinolates. Its gene regulation and synthesis has become a research hotspot. Some gene families（such as MYB, MAM, CYP79/CYP83, and AOP）have played important roles in different stages of glucosinolate synthesis. The biological functions of glucosinolates and the regulating and synthesizing genes involved are reviewed and the prospect of their application in crucifer breeding is also discussed in this paper.
