Cloning of the Genes Resistant to Phytophthore Capsici in Pepper by Using the Differential Display Technique
Yi Tuyong; Zhang Baoxi; Xie Bingyan; et al.
College of Bio-safety Science and Technology; Hunan Agricultural University; Changsha 410128; Institute of Vegetables and Flowers; Chinese Academy of Agricultural Sciences; Beijing 100081
Abstract：We cloned two differentially expressed DNA fragments by using DDRT-PCR of the total RNA from the leaves of two pepper lines inoculated with Phytophthora capsici.We sequenced the fragments after cloning them into a T-vector and BLAST analysis indicated they came from two novel genes.The sequences are available from GenBank as accession AY497910 and AY497911.
收稿日期: 2005-01-10
引用本文:
易图永;张宝玺;谢丙炎;高必达;. 利用差异显示技术克隆辣椒抗疫病相关DNA序列的研究[J]. 中国蔬菜, 2005, 1(12): 11-13.
Yi Tuyong;Zhang Baoxi;Xie Bingyan;et al.. Cloning of the Genes Resistant to Phytophthore Capsici in Pepper by Using the Differential Display Technique. , 2005, 1(12): 11-13.
