Studies on Construction of Bitter Gourd Core Collection Based on SSR Markers and Phenotypic Traits
CUI Junjie1，CHENG Jiaowen2，CAO Yi1，HU Kailin2*
1School of Food Science and Engineering，Foshan University，Foshan 528225，Guangdong，China；2 College of Horticulture，South China Agricultural University，Guangzhou 510642，Guangdong，China
Abstract：Taking advantage of 21 bitter gourd SSR markers and 9 phenotypic traits，this paper constructed core collection for 184 bitter gourd germplasm resources from various geographical origins；and extracted 34 accessions as core collection by combining M strategy sampling method of genotype data and random sampling method of phenotype data．These 34 bitter gourd core collection had reserved 93.46% of the alleles and most of the geographical origins of the initial germplasm．The paper also carried out evaluation on the representativeness of SSR genotype．The results showed that there were no significant differences in the 6 genetic diversity parameters between the core collection and the initial collection．They were the number of alleles（Na），effective number of alleles（Ne），Shannon’s Information index（I），heterozygosity（H），gene diversity（GD） and polymorphism information content（PIC）．The evaluation on the phenotypic representativeness of core collection howed that the mean difference percentage（MD%） was 0，and coincidence rate（CR%）was 88.30%．The above results indicated that the 34 core collection of bitter gourd could represent the diversity of initial collection in terms of genotype and phenotype，meeting the requirement of core collection construction．
崔竣杰 1, 程蛟文 2, 曹毅 1,胡开林 2*. 基于 SSR 标记和表型性状构建苦瓜核心种质的研究[J]. 中国蔬菜, 2022, 1(2): 25-32.
CUI Junjie1，CHENG Jiaowen2，CAO Yi1，HU Kailin2*. Studies on Construction of Bitter Gourd Core Collection Based on SSR Markers and Phenotypic Traits. China Vegetables, 2022, 1(2): 25-32.
