Abstract：Taking okra variety‘Zhunong No.2’as test material，and using Illumina HiSeq TM2500 high-throughput sequencing technology，this paper studied the different expression of transcriptome genes（DEGs） under 400 mmol · L-1 NaCl stress．The results showed that there were 70.17 Gb Clean data from the overground part treated with NaCl and the contrast after 24 hours．The base percentages of Q30 were all over 93.21%．There were 1 396 DEGs gained，including 588 up-regulated genes and 808 down-regulated genes．Among them，1 266 genes were annotated．The GO，KOG and KEGG were annotated according to the sequence of Unigene library．The antioxidant and reactive oxygen scavenging in plants-related genes，including peroxidase 55 gene，superoxide dismutase gene，glutathione S-transferase gene，cinnamate-4-hydroxylate-like gene，flavonol synthase gene and chalcone synthase 2 gene，plant growth and developmentrelated
genes，including WRKY21 gene，ERF2 gene，NAC29 gene，GATA22 gene，MYB4 gene and auxin responsive protein gene，plant osmotic regulation-related genes，including trehalose-phosphatase gene and phosphoethanolamine N-methyltransferase 1 gene．All of them were up-regulated expression．
