Taking virus-free Jiading garlic as test material，this paper studied the effects of water and fertilizer supply on the growth of tissue-cultured seedlings and bulbs and quality of the original seed，and the effects of subculture number and sowing time of tissue-cultured bulbs on quality of the original seed．Then，a production comparative experiment of virus-free original seed and conventional garlic seed was conducted．The optimum combination of treatments（tissue type：tissue culture bulb，32 hole disc，irrigation time of 30 min，fertilizer interval of 14 days）was screened out by water and fertilizer supply experiment．Compared with the 8-generation tissue-cultured bulbs，15-generation tissue-cultured bulbs were more suitable for the original breeding of Jiading garlic．Relative late sowing was beneficial to increase the proportion of normal lobes in the original seed bulbs，but it would significantly affect the accumulation of bulb biomass．In production comparative experiment，various growth index and bulb quality of virus-free garlic seed were remarkably superior than those of conventional garlic seed．Results of this experiment have provided technical support for realizing the industrialization of virus-free seedlings of Jiading garlic．