Genotype identification is an important link in molecular-marker-assisted breeding and genetic
analysis of gene function.Since this method is used more and more frequently,researchers need a high-throughput
technique.This experiment took a pair of SSR primers of complementary type as the method,and compared
the effect of extracting DNA from cotyledon and root during seedling stage.The effect of extracting DNA from each
tissue of the plant by NaOH-boiling method during maturing stage was also verified.The results indicated that
extracting DNA from root with NaOH-boiling method was a rapid way to measure seed purity.Therefore,extracting
DNA with NaOH-boiling method from all tissues of the matured plant could be used for screening plant genotype.
李委,戴祖云,夏伟伟,王雯雯. 一种快速提取南瓜大群体基因组DNA 的方法[J]. 中国蔬菜, 2017, 1(9): 36-40.
LI Wei,DAI Zu-yun,XIA Wei-wei,WANG Wen-wen. A Rapid DNA Extraction Method for Large Squash Population. China Vegetables, 2017, 1(9): 36-40.