Abstract： In this study，methods for identifying cucumber germplasm tolerance to low temperature at germination and seedling stages were compared systematically. First， the cold tolerance of 15 materials were identified under 13 ℃ and 15 ℃（CK：26 ℃），respectively using 6 indexes including germination percentage，
germination power，germination index，radicle length， fresh weight and dry weight of seed buds. The results
showed that 13℃ was suitable for screening materials with strong tolerance to low temperature，while 15 ℃ was
suitable for evaluating cold tolerance of cucumber germplasm resources at budding stage. In addition， different
treatments with stress duration under low temperature at the seedling stage were analyzed and compared taking
injury index and recovery index as evaluation indexes. Treating cucumber seedlings under 6 ℃ constant low
temperature， we found that 3-5 days were the suitable treatment of stressed time. While under 15℃ /5℃ variable
temperature conditions， we found the suitable treatment of stressed time was 9-15 days. Taking recovery index as
evaluation index to identify seedling tolerance to low temperature， we found that the days under stress condition
could be 1-2 days shorter than that taking injury index as evaluation index.
