There is a direct or indirect relationship between plant trichomes and insect resistance，drought tolerance，salt tolerance，disease resistance and ultraviolet radiation，which has important and potential utilization values in resistance breeding．Taking high generation inbred lines of pastel‘1789M’，no pastel chili‘1788W’and their F1、F2 population as the test materials，this experiment observed the morphological characteristics，distribution characteristics of pepper surface trichomes，and studied the genetic regulation of trait and fixed the gene combining the segregation population analysis（BSA）．The results showed that the pepper trichomes are formed part of the epidermal cells jest upward，pepper is given priority to with upright no branch fuzz and density is different in different parts of the plant，generally young stem ＞ matured stem ＞ positive leaf ＞back leaf. We found a pair of dominant nuclear genes controlled pepper trichomes formation，and the pepper gene location was on chromosome 10，the SSR markers between ssr10-19 to ssr10-21．The genetic distance between these 2 markers and the trichomes gene were 5.3 cM and 13.3 cM，respectively．