Molecular Identification on Mixed Infections of Tomato chlorosis virus and Tomato
yellow leaf curl virus
ZHAO Li-ming1， LI Gang1， LIU Yong-guang2， GUO Jia-jin3， WEI Jia-peng3， ZHU Xiao-ping1*
（1 College of Plant Protection， Shandong Agricultural University， Tai’an 271018， Shandong，China；2Institute of Vegetables
and Flowers， Weifang University of Science & Technology， Shouguang 262700， Shandong，China；3 Provincial Engineering
Research Centre for Vegetables of Shandong， Shouguang 262700， Shandong，China）
Abstract： In the autumn of 2013， leaves of greenhouse-grown tomato plants showed symptoms of interveinal
chlorosis， and together with stunted， marginal curling and crumpling. As the disease progressed， leaves become
thick and crisp， similar to symptoms induced by Tomato chlorosis virus（ ToCV） and Tomato yellow leaf curl
virus（ TYLCV） were collected from greenhouse-grown tomato plants in Shouguang， Shandong Province. The
specific primer pairs of CP-F/CP-R specific to ToCV and TY1-R/TY1-F，TY2-R/TY2-F，TY3-R/TY3-F
specific to TYLCV were used to detect ToCV and TYLCV， respectively. 774 bp（ GenBank accession number：
KC709510） and 2 781 bp（ GenBank accession number： KJ546418） specific fragments were amplified from the
same symptomatic samples. Sequence analysis of the amplified sequence of KC709510 showed 99.6% nucleotide
sequence identity with ToCV-BJ（ KC311375） isolates and the sequence of KJ546418 showed 99.6% nucleotide
sequence identity with TYLCV-SDWF-L7（ KC999850） isolates registered in the GenBank.
