Cloning and Expression Analysis of Transcription Factor CsCBF3 Gene from
Cucumber
NING Yu1，MIAO Yong-mei1，2，LI Ji1，LOU Qun-feng1，WENG Yi-qun3，CHEN Jin-feng1*
(1State Key Laboratory of Crop Genetics and Germplasm Enhancement， College of Horticulture， Nanjing Agricultural
University， Nanjing 210095， Jiangsu， China； 2College of Life Science， Anhui Science and Technology University，Fengyang 233100， Anhui， China；3 Horticulture Department， University of Wisconsin， Madison 53706， USA）
Abstract：In order to determine its role in cold response in cucumber（ Cucumis sativus
L.），CsCBF3 was cloned from Chipper using RT-PCR method and its GenBank accession number
was JQ900769．CsCBF3 had an open reading frame of 615 bp，which was supposed to encode
a protein of 204 amino acids．Phylogenetic tree analyses showed that CsCBF3 belonged to CBF
gene family and had close relationship with CBF3 from Vitis amurensis，while had far relationship
with CBF3 from Lolium perenne and Oryza sativa．Bioinformatics analysis showed that CsCBF3
protein was consisted of AP2 DNA binding domain，which included amino acid residues，nuclear
localization signal region and acidic activation domain，and some phosphorylation sites and protein
secondary structures may play important roles in process of interactions between proteins and
DNA．Quantitative real-time PCR results showed that expression of CsCBF3 could be induced
by low temperature，and the level reached to peak very fast and then decreased．These results
suggested that CsCBF3 was a rapid response gene and played an important role in resistance to low
temperature in cucumber.
