枯草芽孢杆菌可湿性粉剂防治黄瓜灰霉病药效试验
曹春娜1,2 ，石延霞2 ，李宝聚2*
1沈阳农业大学植物保护学院，辽宁沈阳110161；2中国农业科学院蔬菜花卉研究所，北京100081
Efficacy Trials of Bacillus Subtilis WP against Cucumber Gray Mold(Botrytis cinerea )
CAO Chun-na1,2 ,SHI Yan-xia2 ,LI Bao-ju2*
1College of Plant Protection,Shenyang Agricultural University,Shenyang 110161,Liaoning,China;2Institute of
Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China
摘要 采用室内生物活性测定及田间药效试验，以40 %嘧霉胺悬浮剂为对照药剂，评价了生物杀菌剂枯草芽孢杆菌可湿性粉剂（活
芽孢数为1 000亿·g-1 ）对黄瓜灰霉病的防治效果。试验结果显示，枯草芽孢杆菌可湿性粉剂对黄瓜灰霉病的抑制效果较好，与对照
药剂相当，其EC50 为5.89×106 cfu·mL-1 。田间试验中枯草芽孢杆菌可湿性粉剂0.08 g·m-2 施药后的防效为70.81 %，对照药剂
0.11 g·m-2 的防效为77.40 %。室内生物活性测定结果与田间试验结果一致。
关键词 ：
黄瓜灰霉病 ,
枯草芽孢杆菌 ,
嘧霉胺 ,
药效试验
Abstract ：The antifungal effect of 1×1011 cfu·g-1 Bacillus Subtilis WP against cucumber gray mold(Botrytis
cinerea )tested by allied toxicity measurement and field efficacy trials.The result showed that Bacillus Subtilis WP
had good inhibitory effect on cucumber gray mold(Botrytis cinerea )with EC50 of 5.89×106 cfu·mL-1 ,which
indicated that it had the same effect with control insecticides Pyrimethanil.The same result was found in field
experiment.The efficacies of 0.08 g·m-2 Bacillus Subtilis and 0.11 g·m-2 control insecticides were 70.81 % and
77.40 %,respectively.
收稿日期: 2008-12-16
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