Analysis on ToCV and TYLCV Co-infection Ratio and Sequence Phylogeny in 6 Provinces of China
HU Rong1，FENG Li-xiao1，ZHANG Yu1，ZHANG Song-bai1，2，ZHENG De-yong1，2，LIU Yong1，2*
1Longping Branch of Graduate School，Hunan University，Changsha 410125，Hunan，China；2Plant Protection Institute，Hunan Academy of Agricultural Science，Changsha 410125，Hunan，China
Abstract：From 2017-2019，in major tomato producing areas of 6 Chinese provinces，tomato samples suspected to be infected by tomato chlorosis virus（ToCV） and（or） tomato yellow leaf curl virus（TYLCV）were collected．This paper conducted RT-PCR or PCR by specific primers，then carried out phylogenetic analysis on PCR products conforming to expected band size after sequencing．Results showed that the recombination co-infection ratio of ToCV and TYLCV from 6 Chinese provinces was 25.29%，among them those of 2 virus from Shandong Province was the highest-46.43%．Results of sequences analysis indicated that the homology of measured ToCV CP gene sequence with reported corresponding sequence was over 98.30%．There were no obvious genetic differentiation in ToCV CP gene sequences．The homology of partial sequence of TYLCV genome with published corresponding sequence was over 96.00%，and no distinct genetic differentiation was occurred．
胡荣，冯丽肖，张宇，张松柏，张德咏，刘勇. 我国6个省份番茄褪绿病毒和番茄黄化曲叶病毒复合侵染率及序列系统发育分析[J]. 中国蔬菜, 2020, 1(9): 31-35.
HU Rong，FENG Li-xiao，ZHANG Yu，ZHANG Song-bai，ZHENG De-yong，LIU Yong. Analysis on ToCV and TYLCV Co-infection Ratio and Sequence Phylogeny in 6 Provinces of China. China Vegetables, 2020, 1(9): 31-35.
