1Biotechnology & Germplasm Resources Institute，Yunnan Academy of Agricultural Sciences，Key Lab of Agricultural
Biotechnology of Yunnan Province，Key Lab of Southwestern Crop Gene Resource and Germplasm Innovation，Ministry
of Agriculture，Kunming 650223，Yunnan，China；2College of Animal Science and Technology，Yunnan Agricultural
University，Kunming 650201，Yunnan，China；3College of Animal Science and Technology，Southwest University，
Chongqing 400715，China）
In order to investigate the response mechanism of Amorphophallus konjac plants growing
under different potassium levels，the total RNA of the leaves of Amorphophallus konjac Fuyuan on the fifth day
after potassium sulfate（low，medium and high level）was applied．Then cDNA was synthesized，and the
differentially expressed genes of Amorphophallus konjac Fuyuan leaves under low，medium and high potassium
sulfate were amplified by optimized cDNA-SRAP，and their bioinformatics was analyzed．The results showed
that 507 gene segments were amplified by 29 pairs primer combinations，including 126 differentially expressed
gene segments．Compared with the medium potassium level，19 inhibited expressed fragments，14 induced
expressed fragments，29 up-regulated expressed fragments，and 64 down-regulated expressed fragments were
found in the low and high potassium levels．After recycling 13 differentially expressed gene segments of good
quantity，9 homologous sequences matched with them in NCBI．Except for 4 nucleotide sequences matching
with higher homology to the known function genes (glutaminyl-tRNA synthetase，β-ATP synthase，YchFGTPbinding
protein YchF，ribonucleotide reductase) and 5 nucleotide sequences matching with higher homology to the
assumption genes or the predict genes，other differentially expressed gene segments were not found，which might
be some new genes of Amorphophallus konjac Fuyuan associated with potassium nutrition uptake．The specific
function of the new genes will be further studied and analyzed in the future．
