Abstract：Taking cucumber（Cucumis sativus L.） seeds and seedlings as test materials，this
paper studies the characteristics of organic ecological soilless cultivation substrate of cultivation 1
year，cultivation 2 years and cultivation 3 years and effect of substrate extract on seed germination and
seedling growth. The results showed that the dry density，pore water，pH and EC values of cultivation
3 years substrate were increased very significantly compared with that of cultivation 1 year．While the
total porosity and pore of cultivation 3 years substrate were decreased very significantly compared with
cultivation 1 year. After treating cucumber seeds and seedlings with cultivation 3 years substrate extract，
their germination rate，sprout potential，germination index and vigor index were decreased very
significantly compared with that of the CK; and the vertical roots length，hypocotyl radicel length，lat-eral root number，epicotyls and hypocotyl width were also decreased very significantly compared with
that of the CK．The seedling plant height，overground fresh weight and dry weight were decreased very
significantly compared with that of the CK；while the fresh weight and dry weight of underground seedlings
were increased very significantly compared with that of the CK. The leaf photosynthetic rate and
water use efficiency of seedlings were decreased very significantly compared with that of the CK．The
leaf stomatal conductance，intercellular CO2 concentration and transpiration rate of seedlings were increased
very significantly compared with that of the CK. The seedling root-shoot ratio，G value，Chlorophyll
a content，Chlorophyll b content，Chlorophyll（a + b） contents and Chlorophyll a/b value were
decreased significantly compared with that of cultivation 1 year substrate extract treatment．Therefore，
after 3 years’cultivation，the substrate characteristics and contents are not favorable fo r cucumber seed
germination and seedling growth．
