College of Horticulture, Northwest A &F University, Key Laboratory of Horticulture Plant Germplasm Utilization in Northwest China, Ministry of Agriculture, Yangling 712100, Shaanxi, China
Abstract：A F2 population from a single plant with obvious heading-splitting hybrid in
Chinese cabbage〔Brassica campestris L. ssp. pekinensis（Lour）Olsson〕, was applied to
analyze the inheritance of head-splitting trait and to screen linkage molecular marker.
The result showed that the best way for surveying head-splitting was delaying plant
ripeness for 20 days. And the separated proportion of no-spliting head to splitting head
is fitted to 3∶1, which means that at least there is a major recessive gene controlling
the trait. In addition，minor genes and environment can also modify the head splitting
degree and speed. In the meantime, the genomic DNA extracted from single plant of F2,
06J31 and 92S24 were used as template to PCR amplification. Primer S15 was selected from
600 random primers and the marker S15-222 was tightly linked with the gene of head-
splitting trait. The linkage analysis showed that the distance between the marker S15-222
and head-splitting gene was 8.876 cM, which is useful to MAS of splitting-resistance in
Chinese cabbage.
