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Current Issue
2014 Vol.1 Issue.12,
Published 2014-12-01
1
FENG Jie-Ming
2014 Vol. 1 (12): 1-3 [
Abstract
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603
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813
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4
ZHAO Dong-Mei
2014 Vol. 1 (12): 4-8 [
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359
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9
Research Progress on Strawberry Anthracnose
HU De-Yu, QIAN Chun, LIU Xue-Feng
The research progress in strawberry anthracnose at home and abroad was expounded comprehensively from classification of strawberry anthracnose， biological characteristics of its pathogen， disease symptoms， occurrence regularity， prevention and control methods. The paper also introduced the current major methods for strawberry anthrax prevention and control from the aspects of agriculture， chemical， biological control and genetic， etc. Before ending， the paper prospected the direction of future research.
2014 Vol. 1 (12): 9- [
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564
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15
Molecular Identification on Mixed Infections of
Tomato chlorosis virus
and
Tomato yellow leaf curl virus
ZHAO Li-Ming, LI Gang, LIU Yong-Guang, GUO Jia-Jin, WEI Jia-Peng, ZHU Xiao-Ping
In the autumn of 2013， leaves of greenhouse-grown tomato plants showed symptoms of interveinal chlorosis， and together with stunted， marginal curling and crumpling. As the disease progressed， leaves become thick and crisp， similar to symptoms induced by
Tomato chlorosis virus
（ ToCV） and
Tomato yellow leaf curl virus
（ TYLCV） were collected from greenhouse-grown tomato plants in Shouguang， Shandong Province. The specific primer pairs of CP-F/CP-R specific to ToCV and TY1-R/TY1-F，TY2-R/TY2-F，TY3-R/TY3-F specific to TYLCV were used to detect ToCV and TYLCV， respectively. 774 bp（ GenBank accession number： KC709510） and 2 781 bp（ GenBank accession number： KJ546418） specific fragments were amplified from the same symptomatic samples. Sequence analysis of the amplified sequence of KC709510 showed 99.6% nucleotide sequence identity with ToCV-BJ（ KC311375） isolates and the sequence of KJ546418 showed 99.6% nucleotide sequence identity with TYLCV-SDWF-L7（ KC999850） isolates registered in the GenBank.
2014 Vol. 1 (12): 15- [
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566
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21
Studies on Inheritance and Molecular Marker in Cabbage Glossy Wax-less Mutant LD10
LIU Dong-Ming, YANG Li-Mei, TANG Jun, LIU Ze-Zhou, FANG Zhi-Yuan, LIU Yu-Mei, ZHUANG Mu, ZHANG Yang-Yong-
To clarify the characteristics and inheritance of cabbage（
Brassica oleracea
L. var.
capitata
L.） glossy wax-less mutant LD10，the major agronomic characteristics and nutrient contents of LD10 were tested， and ultra-structures of leaves in mutant material LD10 and normal material 21-3 were measured，observed and comparatively analyzed. LD10 and 21-3 were taken as parent to construct 6 generations population，and inheritance analysis onthe glossy wax-less characteristics of LD10 was conducted. The results showed that there was little wax on the leaves of LD10，while the wax on the leaves of 21-3 was abundent，and the granular wax in LD10 was of long stick shape，significantly different from the wax in 21-3. The glossy wax-less trait in cabbage LD10 was controlled by one single recessive gene named
cgl-4
. One molecular marker（LT-SSR16）linked to
cgl-4
with genetic distances 4.7 cM. Another F
1
population obtained through crossing LD10 and 10Q-961 showed that the genes leading to glossy wax-less trait were at different locations in the two glossy wax-less materials.
2014 Vol. 1 (12): 21- [
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475
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27
Establishment of Tomato micro-TOM Regeneration System in Vivo
CAO Hui-Ying, WANG Yu-Ying, SUN Jian-Kun, LIU Ling-Li, ZHANG Li-Jun, RUAN Yan-Ye, XIA Run-Xi
Tomato（
Solanum lycopersicum
L.）micro-TOM is a model organism for biology study. Its regeneration system in vitro is of vital importance for genetic transformation and developmental biology study. But tomato micro-TOM tissue culture is not only time-consuming and tedious， but also frequently polluted by microorganism. This experiment introduces a new method of regeneration system in vivo by removing axillary and apical buds of tomato. The method has high speed and efficiency， and does not rely on exogenous hormone. It has layed a foundation for simplifying tomato genetic transformation and provided a good material for plant regeneration mechanism study.
2014 Vol. 1 (12): 27- [
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431
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30
Studies on Planting
Flammulina velutipes
at Chinese Prickly Ash Branch
LI Xiao-Lin, SUN Hai-Yan, LIANG Guo-Lu, HE Qiao, GUO Qi-Gao, XIA Qing-Qing, ZHAO Shen-Qing-Yu
The study about planting
Flammulina velutipes
was conducted by taking Chinese prickly ash branch as raw material．The result showed that the formulation with 45% Chinese prickly ash branch was suitable for
Flammulina velutipes
cultivation. The average yield and biologic efficiency of
Flammulina velutipes
fruiting\ body planted with Chinese prickly ash branch as the main raw material was slightly lower than that planted with the cottonseed hulls as the main raw material．But Ca and Fe contents were significantly higher in the fruiting body grown on Chinese prickly ash branch than that grown on cottonseed hulls．There were no significant differences in the contents of Mg，Cu，Zn， protein， fiber，ash and sugar contents of the fruiting body grown between Chinese prickly ash branch and cottonseed hulls．For the total amino acids contents and essential amino acids，the fruiting body planted with Chinese prickly ash branch were richer than that planted with cottonseed hulls．Chinese prickly ash branch may be used as raw material for planting
Flammulina velutipes
，which was proved to reduce the production cost and improve the ratio of input and output．
2014 Vol. 1 (12): 30- [
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371
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634
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33
Studies on High Mountain Three-dimenstional Climate Resources and Distribution Regulation of High Mountain Vegetable Plantation
QIU Zheng-Ming, LIU Ke-Qun, NIE Qi-Jun, XIAO Wei-Yu, CHEN Lei-Fu
Micro-climate observation and vegetable field experiment were carried out during 2009-2013 in mountains areas of Southwest Hubei at different altitudes. Spatial and temporal variation characteristics of meteorological elements such as temperature， precipitation， evaporation was analyzed. The correlation of yield， quality of pepper， lettuce， and other vegetables， and meteorological conditions were also analyzed. The result showed that in the sustain duration with ≥ 5 ℃、≥ 10 ℃、≥ 15 ℃ the decreasing rate with height increasing per 100 m were 4.8 days，5.5 days，6.8 days，respectively. The precipitation and humidity index increased with height increasing. The fruit setting number of single pepper plant（thermophilic vegetable）and per unit area yield both have significant relation with the duration of days ≥ 15℃ in the flowering and fruiting stage. The contributions of each additional day to single plant fruit setting number and yield per unit were 0.32 and 1.10 t·hm
-2
， respectively. The most suitable planting conditions for warm season vegetables were height between 800-1 200 m， temperature ≥ 15 ℃， sustaining for 100-130 days. There was no summer above 1 600 m hight，where was the suitable planting area for hardy vegetables.
2014 Vol. 1 (12): 33- [
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365
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39
Effect of Soybean Straw Returning to Field on Soil Microbe Quantity under Different Field Moisture Capacity
ZHANG Xue-Yan, TIAN Lei, WU Guo-Hua, ZONG Qing-Shu, GAO Yan-Ming, LI Jian-She
Taking soil for 5 years continnous cucumber cropping as material and utilizing pot culture method， this experiment adopted 4 treatments： no soybean straw returning to the field（CK）； inoculation rhizobium soybean straw returning to the field（T1）； inoculation rhizobium soybean straw with no root nodule returning to the field（T2）； no inoculation rhizobium soybean straw returning to the field（T3），and studied the effect of these treatments on soil sucrase activity and microbe quantity under different field moisture capacity. The results showed that the fungi quantity in T1 was significantly lower than CK in 25％、50％ field moisture capacity for 50 to 140 days，and bacterial quantity maintained a high level in prophase of cultivation（＜ 50 days）. Under 75％ field moisture capacity，actinomyces quantity of the returning treatments was significantly higher than the control. In a word，25％，50％ field moisture capacity could make the bacterial quantity maintaining a high level，and reducing fungus quantity，the returning treatments were better than no returning treatments，inoculation rhizobium treatments were better than no inoculation rhizobium treatments. The actinomyces quantity maintained a high level under 75％ field moisture capacity in late culture stage（＞ 110 days）.
2014 Vol. 1 (12): 39- [
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43
A New Early Maturing Chinese Cabbage F
1
Hybrid —‘Zhongbai 61’
ZHANG Shu-Jiang, LI Fei, ZHANG Shi-Fan, ZHANG Hui, SUN Ri-Fei
‘Zhongbai 61’ is a Chinese cabbage F
1
hybrid with early maturity developed by crossing selfincompatible lines of B80067 and B80032．Its growing period is about 60 days．The plant is semi-erect with compect plant type and green outer leaves. The plant is 36.5 cm in height and 56.0 cm in width．Its head is 27.3 cm in height，15.4 cm in diameter. The head is of short cylinderical shape．The average net head weight is about 2.2 kg．Its net vegetable rate is 72% and head shape index is 1.8. Its yield is about 82.5-90.0 t ·hm-
2
．It is resistant to virus disease，downy mildew and black rot．It has high yield potential and is suitable to be cultivated in Hebei，Shandong， Liaoning Provinces and Beijing in late summer and early autumn.
2014 Vol. 1 (12): 43- [
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383
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45
A New Eggplant F
1
Hybrid — ‘Haifengchangqie No.1’
ZHANG Shu-Gen, HUI Zhi-Ming, WANG Zhen-Quan, XING Yong-Ping, ZHANG Jun-Min, JIANG Zhong-Ren, LI Chun-Ling
‘Haifengchangqie No.1’ is a new long eggplant F
1
hybrid developed by crossing double haploid line 02-QP-19 as female parent and inbred line A-40 as male parent. This variety has strong growth vigor. Its leaf is green，and leaf vein，leaf stalk and main stem are purple in color．The average fruit is 31.5 cm in length，4.5 cm in diameter. The single fruit weight is 220 g．Its fruit peel is black-purple and glossy．The average VC and VP contents are 23.2 mg·kg-1 and 0.035%，respectively. Its yield is about 75 t·hm-
2
．It is suitable to be cultivated in open fields in Beijing，Jilin and Heilongjiang Provinces.
2014 Vol. 1 (12): 45- [
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436
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47
An Early Maturing， Mechanical-harvesting Processing Tomato F1 Hybrid— ‘Xinfan No.48’
ZENG Yi-Hui, XUE Lin, WANG Cheng-Jun, GAO Ming, JIN Feng-Mei, ZHU Yan-Bing, LI Ying, SUN Xiu-Xia-
‘Xinfan No.48’ is an early maturing processing tomato F
1
hybrid developed by crossing F19-3-1- 1 as female parent and TD-06-2 as male parent. It is suitable for mechanical-harvest or manual one-time harvest. It takes 87 days for growing period. The plant is of open type with dark green leaves. The plant is 65 cm in hight with 6-7 branches. The first inflorescence usually sets at the 7th node. Its fruit is of oval shape with shinning deep red color. The fruit shape index is 1.2. Its average single fruit weight is 70-75 g. The lycopenr content is 123 mg·kg
-1
， soluble solid content is 4.64 %， and total acid is 0.38%. Its fruits can stand heavy pressure and tolerant to storage in field condition. Its average yield can reach over 120 t·hm
-2
. It is suitable to be planted in Northern and Southern Xinjiang and Gansu Provinces. It is good for producing tomato paste.
2014 Vol. 1 (12): 47- [
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388
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49
A New Tomato F
1
Hybrid ─‘Hadafen 801’
CHEN Qing-Qi, MEN Wan-Jie, LEI Na
‘Hadafen 801’ is a new tomato F
1
hybrid developed by crossing inbred lines fy-511-nf-111 as female parent and jg-811123 as male parent. This variety belongs to unlimited growth type. The plant has ordinary leaf with compact plant type. It is suitable for close planting. Its fruit is of round shape. The matured fruit is pink in color with equal size. The single fruit weight is about 220 g. It is of early maturity. It is resistant to leaf mold and middle resistant to virus disease. Its yield is about 97.5 t ·hm
-2
．It is suitable for protected field cultivation in North China.
2014 Vol. 1 (12): 49- [
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385
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1491KB] (
514
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51
A New Radish F
1
Hybrid —‘Jingcui No.1’
ZHANG Li, WANG Qing-Biao
‘Jingcui No.1’ is a new radish F
1
hybrid bred by crossing self-incompatible line 05132 and 05177. This variety can be harvested 75-80 days after sowing. The plant is semi-erect with dark green leaves nearly without lobelets. The root is of oval round shape，13 cm in length，12 cm in diameter. The single root weight is 1.2 kg. The depth of root under ground is 1/4 and the exterior color is green and white. The light-green flesh is crispy and juicy. Its VC content is 380 mg·kg
-1
（FW），and glucoslnolate content is 318.8 μmol·kg
-1
（FW）. The yield is 75-90 t·hm-
2
. It has higher resistance to virus disease and soft rot than the cotrol‘Mantanghong’ in field. It is good for eaten raw. This variety is suitable for cultivation in Beijing，Tianjin，Hebei and Shandong Province.
2014 Vol. 1 (12): 51- [
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429
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1841KB] (
536
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54
A New Mini-cucumber F
1
Hybrid—‘Bicui 18’
SHAO Wei-Qiang, JIANG Guo-Jun, ZHENG Ye, ZHU Bi-Yun, WANG Jian-Ke, ZHANG De-Wei
‘Bicui 18’ is a new cucumber F
1
hybrid bred by crossing 0714-2-4-6-3 as maternal parent and 0701-2-2-22-1 as paternal parent.‘ Bicui 18’ has strong growth vigor. The fruit is produced on its main branch. The fruit is in uniform shape and size. It is tolerate to low temperature and weak light. It can bear fruit continuousely and has remarkable high yield potential. The single plant can harvest over 50 melons. Its melon is of short type and is light green in color. The commercial melon is 13.5-15.5 cm in length and about 2.5 cm in diameter. The single fruit weight is 75-85 g. Its melon tastes crisp and light sweet. It is resistant to powdery mildew and Fusiarium wilt， mid-resistant to downy mildew. It can yield over 82.5 t· hm
-2
. It is suitable for protected cultivation in east China， south China and certain areas of central China.
2014 Vol. 1 (12): 54- [
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366
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1533KB] (
441
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56
A New Chinese Kale F
1
Hybrid—‘ Jinlyu’
LI Xiang-Yang, LIU Zhen-Xiang, LIU Zi-Zhu, ZHENG Yan-Song, ZHANG Hua, ZOU Ji-Wen, LI Guang-Guang, HUANG Hong-Di
Jinlyu’ is a Chinese kale F
1
hybrid developed by crossing male sterile line S6-3-2A as female parent，and inbred line G028-3-3 as male parent.‘ Jinlyu’ is an early-medium maturing cultivar. It takes 61 days from sowing to first harvest. The plant has strong growth vigor，erect and compact plant type. Its leaf blades are of oval round shape with dark green color. The leaf stalk is short. Its head is large in size. It tastes crspy and sweet with less fiber. It has good commercial characteristics and superior guality. Its major sprout is 23.5 cm in height，2.3 cm in diameter，and about 165.0 g in weight.‘ Jinlyu’ grows vigorously and is highly resistant to stress. Its resistance to downy mildeu and soft rot is better than the contrast‘ Lyubao’. It can yield 15.5-30.0 t·hm
-2
. It is suitable to be planted all over the China.
2014 Vol. 1 (12): 56- [
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356
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1516KB] (
467
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59
A Loosely Type Cauliflower F
1
Hybrid — ‘Shengsong 60’
JI He-Liang, ZHANG Xin, HAN Nai-Ling, XING Kun, LI Gui-Xiang, DU Yan-Mei, ZHANG Hong-Ying
‘Shengsong 60’ is a F
1
hybrid of spring cauliflower of loosely type developed by crossing S-09-6 as male parent，and self-incompatible line 02-9-9 as female parent. It takes about 60 days from planting in spring to harvest. Its plant has strong growth vigor，and is of compact and erect type. Its leaves are dark green in color with medium wax and short petiole. Its head is white，loose，flat round with long green stems. The head is 25.5 cm in diameter and 13.8 cm in height. The single ball weight is about 1.32 kg. It can yield about 40.5 t·hm
-2
. It is suitable to be cultivated in spring in Hebei，Henan，Shandong，Jiangsu and Liaoning Provinces and Beijing， Tianjin.
2014 Vol. 1 (12): 59- [
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355
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1526KB] (
539
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61
FENG Yu-Qin, ZHANG Yong-Mao, YU Yang
2014 Vol. 1 (12): 61- [
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300
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1577KB] (
695
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65
GUO Xiao-Xian, YU Xiao-Lin
2014 Vol. 1 (12): 65- [
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313
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1594KB] (
457
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69
NONG Ye-Bu-Zhong-Zhi-Ye-Guan-Li-Si
2014 Vol. 1 (12): 69- [
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295
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1447KB] (
404
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70
ZHANG Yu-Xi
2014 Vol. 1 (12): 70- [
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274
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381
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73
JIA Ling-Peng, XIE Xue-Wen, LI Bao-Ju
2014 Vol. 1 (12): 73- [
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346
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1455KB] (
740
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75
LI Shao-Hai, ZHANG Tai-Bao, JIANG Wei-Jie, YU Hong-Jun
2014 Vol. 1 (12): 75- [
Abstract
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347
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2627KB] (
572
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79
XIAO Wan-Li, WU Feng-Zhi, LANG De-Shan
2014 Vol. 1 (12): 79- [
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318
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606
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81
HE Ji-Wen, ZHENG Yu-Feng, CUN Dai-Bin, ZHAO Cong-Xin, WANG Shun-Dang, ZHOU Xue-Jun, YANG Jin-Xi
2014 Vol. 1 (12): 81- [
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315
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1951KB] (
659
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83
CHEN Bin, ZHANG Xiao-Fen, GENG Chuan-Yi, GENG San-Sheng
2014 Vol. 1 (12): 83- [
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312
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1688KB] (
498
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86
LIN Ju-Fen, SONG Xue-Dong, JING Cai-Qiao
2014 Vol. 1 (12): 86- [
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323
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733
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88
YANG Ning
2014 Vol. 1 (12): 88- [
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309
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569
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90
ZHANG Zhong-Mei, ZHANG Gang-Ming, REN Li
2014 Vol. 1 (12): 90- [
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294
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1580KB] (
587
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93
WEI Min, MA Bin-Sheng
2014 Vol. 1 (12): 93- [
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292
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2268KB] (
367
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95
ZHANG Bao-Cai, CUI Lu-Lu, HUA Juan-Li, ZHANG Xiu-Rong
2014 Vol. 1 (12): 95- [
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292
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1729KB] (
495
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96
XU Shu-Lian
2014 Vol. 1 (12): 96- [
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303
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578
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98
2014 Vol. 1 (12): 98- [
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2014 Vol. 1 (12): 99- [
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442
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