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| Establishment of Tomato micro-TOM Regeneration System in Vivo |
| CAO Hui-ying， WANG Yu-ying， SUN Jian-kun， LIU Ling-li， ZHANG Li-jun， Ruan Yan-ye， XIA Run-xi* |
（College of Biological Science and Biotechnology，Shenyang Agricultural University，Liaoning Provincial Research Center
of Plant Gene Engineering Technology， Shenyang 110866， Liaoning，China） |
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Abstract Tomato（ Solanum lycopersicum L.）micro-TOM is a model organism for biology study. Its regeneration
system in vitro is of vital importance for genetic transformation and developmental biology study. But tomato micro-TOM
tissue culture is not only time-consuming and tedious， but also frequently polluted by microorganism. This experiment
introduces a new method of regeneration system in vivo by removing axillary and apical buds of tomato. The method has
high speed and efficiency， and does not rely on exogenous hormone. It has layed a foundation for simplifying tomato
genetic transformation and provided a good material for plant regeneration mechanism study.
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Received: 16 July 2014
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