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Current Issue
2014 Vol.1 Issue.5,
Published 2014-05-01
1
LANG Feng-Qing, CAO Qi-Wei, LI Li-Bin, YANG Ning, SUN Xiao-Lei
2014 Vol. 1 (5): 1- [
Abstract
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412
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1618KB] (
636
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6
Research Progress on
Botrytis cinerea
Resistance to Different Fungicides
HAN Zhi-Qi, BEN Hai-Yan, XIE Xue-Wen, SHI Yan-Xia, LI Bao-Ju
Botrytis cinerea
is a phytopathogenic fungi responsible for grey mould，which is an important disease in many species of fruit trees，vegetables and ornamental crops. Recently，
B. cinerea
has developed increasing severe resistance to a broad range of fungicides. This paper summarizes the research progress about
B. cinerea
at various host plant’s resistance to drugs，their resistance mechanism and methods for detecting tolerant bacteria reported at home and abroad. It also predicts the future orientation of this study.
2014 Vol. 1 (5): 6- [
Abstract
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523
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1KB] [
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1580KB] (
832
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11
Construction of Cherry Tomato Fruit Specific
LYC-B
Interference Vector and Validation of Its Specific Expression in Fruits of Discrepant Colors
WANG Qiao-Li, LIANG Yan, ZHANG Zhen-Cai, LI Cui, LI Yun-Zhou, WANG Ling-Hui
The experiment will be conducted to research the specific impacts of lycopene β-cyclase interference on carotenoid metabolism，cherry tomato（
Solanum lycopersicum
L. var. c
erasiforme
Alef.） fruitspecific lycopene β-cyclase interference vector was constructed，and the validity in different color fruits were verified. Cherry tomato fruit-specific promoter E8 was amplified according to X13437.1 and fruit-specific vector of E8-pBI121 was constructed，which could be expressed in cherry tomato fruits of pink，red，green and purple colors. According to X86452.1，two different lycopene β-cyclase sequences
B
1 and
B
2 were amplified，
LYC-B
1 with 801 bp from 61 bp to 861 bp and
LYC-B
2 with 302 bp from 480 bp to 781 bp of
LYC-B
. The interference vector of pBI121-B1B2 with CaMV 35S promotor and the fruit-specific expression interference vectors of E8- pBI121- B1B2 with E8 promotor were constructed. E8-pBI121，pBI121-B1B2 and E8-pBI121-B1B2 were transferred into
Agrobacterium tumefaciens
GV3101 with the freeze-thaw method，then transformed into living cherry tomato fruits and leaves by injection. The results of GUS staining 7 days after injection showed that E8-pBI121 expressed in pink，red，green and purple cherry tomato fruits；pBI121-B1B2 expressed in leaves，fruits and seeds，while E8- pBI121-B1B2 expressed just in fruits and seeds.
2014 Vol. 1 (5): 11- [
Abstract
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522
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1KB] [
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2066KB] (
723
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19
Toxin-producing Condition Optimization and Biological Activity Determination of
Colletotrichum orbiculare
TANG Shuang-Shuang, LIU Zhi-Heng, YU Chao-Ge, ZHENG Chuan, LI Jian-Bing, ZHAO Ting-Chang
Toxin-producing condition optimization and determination of crude toxin biological activity and its physical，chemical properties were carried out on
Colletotrichum orbiculare
. The results showed that different culture conditions had different influences on pathogenic toxin activity. The Richard culture medium with pH 6 ，culture temperature at 25℃，in dark，and shaking continuously for 20 d were the best toxin-producing conditions. The watermelon stem inoculated by crude toxin could produce the same acute symptom as the bacteria did，which had inhibitory effect on seed radicle growth and seed germination； and also a collapsing role on seedling. That toxin is the main pathogenic factor in the process of watermelon anthrax bacteria infection. Among the 10 kinds of selected plants inoculated by crude toxin，the symptoms of muskmelon，summer squash and strawberry were significant，while no symptoms were seen on tomato，eggplant and pepper.
2014 Vol. 1 (5): 19- [
Abstract
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461
) [
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1KB] [
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1973KB] (
697
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26
SRAP Analysis of Mustard Germplasm Genetic Diversity and Genetic Relationship
LI Ning, SI Jun, REN Xue-Song, SONG Hong-Yuan, LI Cheng-Qiong
Abstract：The paper analyzed the genetic diversity of 111 mustards〔
Brassica juncea
（L.）Czern. et Coss.〕 materials using SRAP markers，and recorded their morphological characteristics．21 primer combinations with high polymorphism were screened from 300 SRAP primer combinations．106 polymorphic bands among 150 bands were amplified by 21 SRAP primer combinations，and the polymorphic rate was 70.67%．The SRAP clustering results showed that the genetic similarity of 111 mustards ranged from 0.38 to 0.89，and 111 mustards were divided into 5 groups and several subgroups．The agronomic traits including leaf blade traits，and root，stem，leaf，bolting development were investigated and analyzed．The results showed that the genetic similarity of 111 mustards ranged from 0.39 to 1.00，and they could be divided into 4 groups and several subgroups．These two results were nearly the same，indicating that SRAP markers could be applied in studying the genetic diversity and relationships of mustard germplasm resources．
2014 Vol. 1 (5): 26- [
Abstract
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486
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1KB] [
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1931KB] (
686
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33
Effects of Saline Stress on Growth and Rhizoplane Soil Enzyme Activities of Cucumber Seedling Grafted on Different Rootstocks
ZHAO Yuan, WU Feng-Zhi
Based on the saline content and composition of the soda saline soil in Heilongjiang and other Provinces，this experiment simulated its saline composition，adopted pot culture，grafted cucumber（
Cucumis sativus
） seedlings onto 2 salt-tolerant rootstocks，and 2 salt-sensitive rootstocks as experimental materials. Ownroot seedlings were taken as the contrast. Saline solution（100 mmol·L
-1
，pH 9.0） was used to treat the soil. We studied the effects of salt stress on growth and soil enzyme activities of cucumber seedlings grafted on these different rootstocks. The results indicated that the yield per plant，the ground fresh weight，the root fresh weight，the plant height and the chlorophyll content of cucumber seedlings grafted on 2 salt-tolerant rootstocks were significantly higher than that of the 2 salt-sensitive rootstocks and own-root cucumber. The soil urease，neutral phosphatase activities after transplanted 30 d and the catalase，dehydrogenase activities after transplanted 40 d of cucumber seedlings grafted on salt-tolerant rootstocks were higher than that of salt-sensitive rootstocks and own-root plants. These 4 kinds of soil enzyme activities of cucumber seedlings grafted on salt-tolerant rootstocks after transplanted 50 d were all higher than that of salt-sensitive rootstocks and own-root cucumber. Under soil salinization condition，cucumber seedlings grafted on salt-tolerant rootstocks grew robustly and suffered low degree of salt injury. That account for that graft can significantly improve cucumber salt resistance； the resistance enhancement of cucumber seedlings grafted on salt-tolerant rootstocks is closely related to the increase of soil enzyme activity.
2014 Vol. 1 (5): 33- [
Abstract
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516
) [
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1KB] [
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1766KB] (
551
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39
Impact of Temperature on Virulence of
Beauveria bassiana
Canada No.1 Isolate to
Bemisia tabaci
（Gennadius）
LIU Zhao, ZHANG Xian-Liang, LEI Zhong-Ren
To identify the effects of temperature on the infection of
Beauveria bassiana
Cananda No.1 isolate against the tobacco whitefly，
Bemisia tabaci
（Gennadius），the spore germination rate，mycelial growth speed，changes in activities of extracellular proteinase and chitinase were observed and studied under 5 different temperature conditions（19，22，25，28，31℃）. The results showed that the isolate Cananda No.1 owned the maximum parameter values and the highest virulence against
B. tabaci
at temperature of 28 ℃ . The spore germination rate，mycelial growth speed，activities of extracellular proteinase and chitinase，and virulence against
B. Tabaci
were significantly reduced when the temperature was higher or lower than 28 ℃ .
2014 Vol. 1 (5): 39- [
Abstract
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438
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1KB] [
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1551KB] (
735
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43
Effects of Copper on Black Sesame Spot and Total Phenol Content in Chinese Cabbage
CHEN Qi-Wu, YANG Xiao-Yun, LUO Qing-Xi, ZANG Shun, ZHANG Shu-Xia, SI Chao-Guang, WANG Yuan, ZHANG Qing-Xia
Taking Chinese cabbage〔
Brassica campestris
L. ssp.
pekinensis
（Lour.）Olsson〕line 03B9 with high sensitivity and C24 with high resistance to black sesame spot as experimental materials，this paper studies the effect of different copper concentration treatments on black sesame spot， and total phenol content and PPO activity. The results showed that the sesame spots of the 2 lines were markedly increased as the copper concentration increased，and the occurrance degree of 03B9 was significantly higher than that of C24. During the copper treatments，the total phenol content，PPO activity in the petiole of line C24 were higher than that in line 03B9 with 25.6% and 77.0%，respectively. The preliminary tests showed that under hydroponic conditions，the occurrence of black sesame spot was promoted by the concentration of exogenous copper, the degree of disorder was related to the copper treatment concentration. Meanwhile, the total phenol content and PPO activity were influenced by copper.
2014 Vol. 1 (5): 43- [
Abstract
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488
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1KB] [
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1678KB] (
601
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47
A New Subgynoecious Autumn Cucumber F
1
Hybrid—‘Tangqiu 208’
HAN Jing-Ling, LI Yu-Hua, SONG Rui-Sheng, HOU Bo-Sheng
‘Tangqiu 208’ is a new early maturing and subgynoecious autumn cucumber F
1
hybrid bred by crossing C18 as female parent and P107 as male parent. Its female flower rate is 85.17%. The commercial fruit is 14.63 cm in length and 3.97 cm in diameter. The average single fruit weight is 147 g. Its fruit skin is green and shinning with larger and dilute tubers. The fruit has good commercial character. Its VC content is 87.0 mg·kg
-1
. Its yield is about 75 t · hm
-2
. It is suitable for protected and open field cultivation in spring and autumn. It is suitable to be planted in Hebei，Liaoning Provinces and Tianjin，etc.
2014 Vol. 1 (5): 47- [
Abstract
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575
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1494KB] (
696
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49
A New Hot Pepper F1 Hybrid—‘Yumeiren’
ZHAO Xiang-Mei, GUO Jing, ZHANG Jian-Guo, CHEN Man, SHEN Ai-Min
‘Yumeiren’ is a mid-early maturing hot pepper F
1
hybrid bred by crossing X-01-7-2-2 as female parent and X-08-3-4-1-1 as male parent. The plant grows vigorously. The first flower sets on the 10.6 node. The young maturing fruit is yellow green and the old matured fruit is bright red with good commercial quality and hot flavor. Its fruit is long lanceolate，with 22.0 cm in length and 1.5 cm in diameter. The single fruit weight is 15- 20 g. Its capsaicin content is 53.60 mg·kg
-1
and the VC content is 895 mg·kg
-1
. It has higher resistance to virus disease and phytophthora blight than that of the contrast‘ Xiangla No. 4’. It can yield 45-60 t·hm
-2
. It is suitable to be grown in protected and open fields in spring and autumn in Henan Province and surrounding areas.
2014 Vol. 1 (5): 49- [
Abstract
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483
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1KB] [
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1485KB] (
446
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52
A New Sweet Pepper F
1
Hybrid—‘Zhangtianjiao No.1’
WANG Ai-Wen, ZHONG Hong-Qing, YAN Ming, XUE Long, HAN Shun-Bin, HUA Jun, SUN Bing-Ling
‘Zhangtianjiao No. 1’ is a new sweet pepper F
1
hybrid developed by crossing inbred line Y03- 167 as female parent and Y03-96 as male parent. It takes 75-85 days from planting to harvesting. The first bud is borne at node 12-14. It is of mid-late maturity. The matured fruit is of square lantern shape，with 3 - 4 lobes， 0.7-0.9 cm in pulp thickness，10-11 cm in length and 9-11 cm in diameter. The matured fruit is green in color with smooth surface. The single fruit weight is 200-260 g. It is tolerant to storage and transportation. It has high fruit setting rate. Its fruit shape symmetry is beautiful. It has good commodity. Its normal yield can reach 81.0 t· hm
-2
. It is suitable for cultivation in open fields at Hexi of Gansu Province and Linhe of Inner Mongolia Autonomous Region.
2014 Vol. 1 (5): 52- [
Abstract
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500
) [
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1KB] [
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1526KB] (
425
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54
A New Muskmelon F
1
Hybrid—‘Dunmi No. 1’
MA Wen-Hai, XI Xiao-Fei, XU Hui-Xin
‘Dunmi No.1’ is a new muskmelon F
1
hybrid developed by crossing 2 inbreed lines ML29 as female and ML20 as male parent．It is of mid-early maturity. Its growth period takes about 87 days in open field cultivation. The fruit development period is about 40 days. The plant grows vigorously. Its leaf is dark green in color. The fruit shape is oval and index is 1.4. The fruit skin is pure white with overlapping curve. Its pulp is light green in color and the pulp thickness is 3.4 cm. The central soluble solid content is 16.4%. It is suitable for storage and transportation. The single fruit weight is about 1.5 kg and its average yield is about 33.9 t·hm
-2
. It is resistant to melon Fusarium wilt，downy mildew and powdery mildew. It is suitable to be cultivated in Jiuquan and similar areas of Gansu Province.
2014 Vol. 1 (5): 54- [
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465
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1KB] [
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1477KB] (
517
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57
KONG Ling-Juan, ZHANG Rui-Ming, SHEN Hai-Bin, ZHU 恩
2014 Vol. 1 (5): 57- [
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322
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1527KB] (
516
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59
YUAN Wen-Jun
2014 Vol. 1 (5): 59- [
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333
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1703KB] (
407
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61
NONG Ye-Bu-Zhong-Zhi-Ye-Guan-Li-Si
2014 Vol. 1 (5): 61- [
Abstract
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336
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1KB] [
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1408KB] (
378
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62
ZHANG Yu-Xi
2014 Vol. 1 (5): 62- [
Abstract
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315
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1KB] [
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1464KB] (
284
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65
TIAN Hui, XIE Xue-Wen, LI Huan-Ling, WEI Song-Hong, LI Bao-Ju
2014 Vol. 1 (5): 65- [
Abstract
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335
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1KB] [
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1426KB] (
667
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67
LIU Yong-Guang, WEI Jia-Peng, QIAO Ning, LI Mei-Qin, LIU Xiao-Ming, ZHU Xiao-Ping
2014 Vol. 1 (5): 67- [
Abstract
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481
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1KB] [
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879KB] (
1177
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70
LIU Cai-Ling, MIAO Feng, LAI De-Qiang, ZHANG Wen-You
2014 Vol. 1 (5): 70- [
Abstract
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321
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1534KB] (
430
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72
MA Hai-Yan, HAO Guo-Fang, HAN Wen-He, CHEN Qiang
2014 Vol. 1 (5): 72- [
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327
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1KB] [
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1826KB] (
600
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74
ZHENG Yuan-Hong, SUN Chuan-Chuan, CHEN Zu-Yao, LI Qi-Hua, MOU Dong-Ling
2014 Vol. 1 (5): 74- [
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340
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1950KB] (
585
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76
FAN Dong-Long
2014 Vol. 1 (5): 76- [
Abstract
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312
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1907KB] (
634
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79
CHEN Nai-Chun, JIN Hua, WAN Li-Ying
2014 Vol. 1 (5): 79- [
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300
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1590KB] (
425
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81
YANG Dong-Yan, YANG Zi-Qiang, YANG Chang-Xin, HAN Ji-Shan, LI Xiu-Fang, GUO Wen-Zhong
2014 Vol. 1 (5): 81- [
Abstract
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312
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1552KB] (
354
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84
YIN Ru-Song, WANG Pei-Quan, GAO Hui-Min, SONG Li-Yan, ZHANG Wen-You, HAN Jun-Xia, WANG Juan
2014 Vol. 1 (5): 84- [
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320
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1553KB] (
531
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87
《Zhong-Guo-Shu-Cai-》Bian-Ji-Bu
2014 Vol. 1 (5): 87- [
Abstract
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344
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1693KB] (
438
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90
CHANG Jian-Wu, WANG Yan, HAO Xiao-Peng
2014 Vol. 1 (5): 90- [
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347
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2140KB] (
378
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92
LIN Chao-Hui, DI Ying-Fen, SITu Zhi-Mou
2014 Vol. 1 (5): 92- [
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329
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1450KB] (
355
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