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| Construction of Cherry Tomato Fruit Specific LYC-B Interference Vector and
Validation of Its Specific Expression in Fruits of Discrepant Colors |
| WANG Qiao-li，LIANG Yan*，ZHANG Zhen-cai，LI Cui，LI Yun-zhou，WANG Ling-hui |
| College of Horticulture，Northwest Agriculture & Forestry University，Yangling 712100，Shaanxi，China |
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Abstract The experiment will be conducted to research the specific impacts of lycopene β-cyclase
interference on carotenoid metabolism，cherry tomato（Solanum lycopersicum L. var. cerasiforme Alef.） fruitspecific
lycopene β-cyclase interference vector was constructed，and the validity in different color fruits were
verified. Cherry tomato fruit-specific promoter E8 was amplified according to X13437.1 and fruit-specific vector
of E8-pBI121 was constructed，which could be expressed in cherry tomato fruits of pink，red，green and purple
colors. According to X86452.1，two different lycopene β-cyclase sequences B1 and B2 were amplified，LYC-B1
with 801 bp from 61 bp to 861 bp and LYC-B2 with 302 bp from 480 bp to 781 bp of LYC-B. The interference vector
of pBI121-B1B2 with CaMV 35S promotor and the fruit-specific expression interference vectors of E8- pBI121-
B1B2 with E8 promotor were constructed. E8-pBI121，pBI121-B1B2 and E8-pBI121-B1B2 were transferred
into Agrobacterium tumefaciens GV3101 with the freeze-thaw method，then transformed into living cherry tomato
fruits and leaves by injection. The results of GUS staining 7 days after injection showed that E8-pBI121 expressed
in pink，red，green and purple cherry tomato fruits；pBI121-B1B2 expressed in leaves，fruits and seeds，while
E8- pBI121-B1B2 expressed just in fruits and seeds.
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Received: 26 December 2013
Published: 10 March 2014
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