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  Current Issue
 
2013 Vol.1 Issue.10,Published 2013-05-03
1 Studies on Applying Solanum torvum Sw. in Eggplant Graft
LI Nan-Yang, CHEN Yu-Hui, LIU Fu-Zhong, ZHANG Ying, LIAN Yong
Solanum torvum Sw. which is relatived wild eggplant with good disease resistance and
stress tolerance，is widely used as rootstock in Solanaceae crops cultivation．It has disease resistance
and can increase production．The article reviews the research progress in applying S. torvum as
rootstock in eggplant production，and studying the mechanism of resisting disease and overcoming stress
injury by graft cultivation.
2013 Vol. 1 (10): 1-8 [Abstract] ( 1336 ) [HTML 1KB] [ PDF 943KB] ( 3613 )
9 Research Progress in Seed Treatment
Mao-Lian-Gang, YAN Dong-Dong, WU Zhuan-Fang, MA Tao-Tao, WANG Qiu-Xia, LI Yuan, GUO Mei-Xia, OuYang-Can-Bin , CAO Ao-Cheng, ZHENG Jian-Qiu
Seed treatment is an economical and effective method in plant disease control．It
adopts biological，physical，chemical technology to protect seed and plant，control the diseases and
pests，insure the normal growth of crops，and achieve superior quality and high yield．According to
the development history of seed treatment technology and differences between various seed treatment
technology，this paper reviews the concepts，principles，classification，characteristics and
application，so as to provide scientific guidance for seed treat ment in actual production.
2013 Vol. 1 (10): 9-15 [Abstract] ( 818 ) [HTML 1KB] [ PDF 885KB] ( 1365 )
16 Impact of External Shock on Fluctuation of Vegetable Price　—Based on Inter-provincial Panel Data VAR Model
LUO Chao-Ping, LI Wei-Yi, Zhai Qiong
On the basis of building panel vector auto-regression model（PVAR），the authors
empirically analyzed the impact of external shock on fluctuation of vegetable price；and found that
except GDP growth rate，which had a significant impact on price fluctuation，other variables contributed
only 1.5%-3.3% to price variation. Meanwhile，considering the impact from variable，when given the
subsidies-LBT a standard differential impact，the vegetable price-LJG would response negatively and
reach a maximum in the third period．When given the vegetable disaster area LCZ a standard deviation，
the vegetable price - LJG would initially turn a negative response then to positive response in the third
period. When given GDP growth a deviation shock，vegetable price-LJG showed a distinct positive
response．When given the LCZSR a deviation shock，vegetable price-LJG showed negative response．
Therefore，the effect of external shock to vegetable price is not ideal，there exists hysteresis qualityin its response to partial external shock. Consequently，building a sound vegetable industry market
mechanism，improving the efficiency of vegetable circulation，and ensuring the balance between
vegetable supply and demand are important measures to inhibit t he violent fluctuation of vegetable price.
2013 Vol. 1 (10): 16-22 [Abstract] ( 733 ) [HTML 1KB] [ PDF 943KB] ( 825 )
23 Distribution of S-haplotypes in Cabbage F1 Hybrid
MIAO Wen-Wen, TIAN Lei, ZHUANG Mu, LIU Yu-Mei, YANG Li-Mei, ZHANG Yang-Yong, FANG Zhi-Yuan
DNA fragments corresponding to kinase domain of S locus kinase（ SRK） gene were
amplified by PCR with 3 pairs of specific primers in all of the 23 cabbage（Brassica oleracea L. var.
capitata L.） F1 hybrids．The S haplotypes of cabbage F1 hybrid were preliminary identified via DNA
sequencing and Blast analysis．The results indicated that 11 S haplotypes were found in cabbage F1
hybrid，including class Ⅰ haplotypes，S7，S16，S28，S35，S45，S57，S68 and an unknown one
named Sn，and class Ⅱ haplotypes S2，S5 and S15．The highest frequency of S haplotyes was S5 with
19.6% and followed by S28 with 17.4% in this study．Compared over 50 S haplotypes in Brassica，the
11 S haplotypes in cabbage F1 hybrid suggests that it is very necessary for exploiting more S haplotypes
in cabbage breeding programs.
2013 Vol. 1 (10): 23-28 [Abstract] ( 734 ) [HTML 1KB] [ PDF 1221KB] ( 852 )
29 Construction and Expression of a Cucumber Lipoxygenase Gene CsLOX2 in
Prokaryotic Cells
CHEN Qiao, CHEN Shu-Xia, WANG Cong-Ying, HAO Li-Ning, WAN Xu-Hua, SHEN Xiao-Qing, CHENG Zhi-Hui
The ORF of cucumber（Cucumis sativus L.）CsLOX2 gene was inserted into an
expression vector pGEX-4T-1 through PCR method using specific primers Yh1 and Yh2，and the
recombined expression vector p4t-LOX2 was constructed. The expression amount of fusion protein
was detected under different expressive conditions，such as different time，different temperature and
different concentration of IPTG. The 115 kD fusion protein was obtained and induced by IPTG and it
showed that CsLOX2 gene encoded a 89 kD protein after the 26 kD GST protein tag was removed．The
proper induction condition was 37 ℃ temperature，0.80 mmol·L-1 IPTG concentration and 10.5 h
inducing time.
2013 Vol. 1 (10): 29-35 [Abstract] ( 805 ) [HTML 1KB] [ PDF 1368KB] ( 849 )
36 Preliminary Study on Cold-induced Transcription Factor CBF3 Transformed
in Eggplant
MENG Ping-Hong, WAN Fa-Xiang, WANG Yong-Qing, WEN Lin-Hong, GUO Jing-Tao, SU Cheng-Gang
We successfully constructed cold-induced transcription factor CBF3（CRT binding
factor），which was transferred to eggplant‘ Sanyueqie’（ Solanum melongena L.）by agrobacteriummediated
transformation. And we obtained 2 transgenic eggplant plants containing cold-tolerant
gene CBF3，through Polymerase Chain Reaction（PCR）detection genomic DNA，and kanamycin
solution daubing on the leaves of generation plants in the field confirmed that the transgenic plants of
eggplant were positive．Thus，we successfully established regeneration and transformation systems
in eggplant.
2013 Vol. 1 (10): 36-43 [Abstract] ( 757 ) [HTML 1KB] [ PDF 2236KB] ( 857 )
44 Initial Studies on Defense Gene Expression Mechanism of Phaseolus vulgaris L.
var. chinensis Hort. to Anthracnose at Seedling Stage
HU Guo-Fu, XIAO Zi-Heng, ZENG Leng-Nan, ZHANG Ting, LI Cheng-Yan
Use susceptible cultivar‘Laolaishao’and resistant cultivar‘Dayoudou’as test
materials，the relative expression of 5 defense enzyme genes（chitinase， β-1，3-glucanase，SOD，
POD and LOX gene）and HRGP protein gene were detected and analyzed by real-time PCR after 2
cultivars infected by anthracnose in different times（ 0，3，6，9，12 d）. The results showed that in
addition to the β-1，3- glucanase gene and SOD gene，the other 4 genes expression were higher in
resistant varieties than the susceptible varieties before infected；after infected chitinase gene and the
LOX gene expression showed decreased-increased trend in susceptible cultivar，and resistant varieties
reached the peak，when susceptible varieties decreased to the minimum（3 d），the relative expression
of resistant varieties was higher than that of the susceptible ones in the whole stage（0-12 d）；β-1，
3-glucanase gene，POD gene and SOD gene were in the same trend，but the expression of resistant
varieties was higher than the susceptible varieties.
2013 Vol. 1 (10): 44-49 [Abstract] ( 760 ) [HTML 1KB] [ PDF 1626KB] ( 843 )
50 Evaluation about Chlorpyrifos Residue in Fruit of Cucumber Germplasm
Resources
YANG Jing, QIN Zhi-Wei, ZHOU Xiu-Yan, XIN Ming
In this experiment，38 cucumber（Cucumis sativus L.） samples of different ecological
types were tested．The chlorpyrifos pesticide was sprayed on cucumber samples during the full fruit
period．Then we tested the samples with gas chromatography，in order to evaluate the chlorpyrifos
residue in the main cucumber germplasm resources．The results showed that there were great
differences between different samples fruits in the level of chlorpyrifos residue. The chlorpyrifos residue
is 0.795 mg·kg-1 on average，the change is between 0.072-1.835 mg·kg-1，and the CV is 0.549．
Furthermore，different chlorpyrifos residue levels exists in cucumbers fruits of different ecological types
and cucumbers from different producing areas. Only one sample，with chlorpyrifos residue lower than
the national standard 0.1 mg·kg -1（GB2763—2005），was selected.
2013 Vol. 1 (10): 50-55 [Abstract] ( 708 ) [HTML 1KB] [ PDF 991KB] ( 617 )
56 Effects of Different Bio-organic Fertilizer Application Rates on Controlling
Bitter Gourd Fusarium Wilt，Soil Microorganisms and Enzyme Activities
REN Shuang, LIU Ying, CAO Qun, DING Wen-Juan, HE Yuan-Huan, GONG Qi-Xin, LI Hua-Xing-*
The pot experiment was conducted by using the bio-organic fertilizer（BOF）made
by mixing the mature compost with functional bacteria to study the effects of different applying quantity
of bio-organic fertilizer on controlling bitter gourd（Momordica charantia L.） Fusarium wilt
and on soil microorganisms and enzyme activities．The results indicated that the occurrence of bitter
gourd Fusarium wilt was decreased significantly by applying BOF．The disease-control effects
of the 3 BOF treatments reached 22.21%，38.91% and 44.45%，respectively，when the diseasecontrol
effect of CF was 0. With the same effective nutrient，the plant stem diameter，leaf length
and leaf width，and plant dry weight under BOF2 treatment were significantly higher than that of CF
treatment，with remarkable growth stimulating effect．The number of bacterium and actinomycesin soil were increased by BOF，while the amount of fungi and Fusarium oxysporum in soil were reduced
evidently．Application of BOF could increase the activities of soil urease，phosphatase and
invertase，which were higher than those of CF treatment．But the change of catalase was uncertain．
The results showed that BOF could improve soil microbial flora and enhance soil enzyme activity，improve
soil environment condition，and prevent the occurrence of Fusarium wilt.
2013 Vol. 1 (10): 56-63 [Abstract] ( 962 ) [HTML 1KB] [ PDF 999KB] ( 753 )
64 Effects of Mixed Corn Stalk Substrates on Growth and Photosynthesis of
Eggplant Seedlings
SU Li-Ying, SONG Shu-Yao, ZHAO Chun-Bo, ZHANG Xue-Mei, ZHAO Jing, ZHANG Yue
Taking eggplant（Solanum melongena L.）cultivar‘Shenqie No.1’as experiment
material，and traditional peat and vermiculite as contrast，this study mixed corn stalk substrate with
different sizes zeolite in various rations as seedling substrates and studied the physical and chemical
properties of this substrates and its applying effect on eggplant seedling cultivation．The results showed
that adding zeolite to seedling substrate could increase the water holding porosity，and decrease in its
pH value and electrical conductivity（ EC）．When zeolite addition reached 20%-40%，the physical
and chemical properties of this substrate could meet the requirement of seedling culture and make the
emergence rate，growth vigor，and quality of eggplant seedling superior than that of the contrast. The
media（ corn stalk and zeolite ratio is 70V∶30V，zeolite in 10 mesh） could improve the quality of
eggplant seedling．Its total chlorophyll content and photosynthetic parameters of eggplant seedling leaf
were the highest，and the strong seedling index was significantly higher than those of the control and
other treatments.
2013 Vol. 1 (10): 64-70 [Abstract] ( 795 ) [HTML 1KB] [ PDF 947KB] ( 724 )
71 Effect of Different Wall Materials on Temperature Effect of Greenhouse
LIANG Nan, LU Zhi-Quan, JIANG Xin-Mei, YU Xi-Hong, WU Feng-Zhi
This research used -40℃ outdoor temperature as designing standards and red brick wall
as the contrast to study the effects of different wall materials on temperature effect of greenhouse．The
results showed that the greenhouse wall structure using 240 mm，20 eyelets perforated brick as interior
wall，120 mm XPS as external wall and 25 mm cement mortar as outermost layer，had higher endothermic
and exothermic capacity，and could raise the soil and air te mperatures effectively.
2013 Vol. 1 (10): 71-75 [Abstract] ( 663 ) [HTML 1KB] [ PDF 945KB] ( 652 )
76 Effect of Different Treatments on Seed Germination of Water Shield
（Brasenia schreberi J. F. Gmel）
LI Shuang-Mei, KE Wei-Dong, ZHU Hong-Lian
Using water shield（Brasenia schreberi J.F.Gmel）newly collected seed as material，
the paper studied on the effects of different treatments on the germination rate and energy．The results
showed that 4 °C low temperature treatment had better effect on improving the germination rate of water
shield seed．It could get better effect to break dormancy of water shield seeds with the treatment of 4 °C
cold water storage，soaking with 500 mg·L-1 GA3 for 6 h and then in 40 °C water for 50 min．25 °C was
the best germination temperature．Under the condition of 20 °C, the seed would take long germination
cycle，and under the condition of 30 °C，the seed was easy to breed white rot around germination hole．
Under partial lighting conditions，seed had a higher germination rate，and almost no germination under
dark condition．
2013 Vol. 1 (10): 76-79 [Abstract] ( 1013 ) [HTML 1KB] [ PDF 908KB] ( 713 )
80 Headspace Sampling GC-MS Analysis of Volatile Flavor Components in
Garlic Scape
WANG Chang-Zhu, GAO Jing-Cao, MENG Huan-Wen
The volatile flavor components in garlic（Allium sativum L.） scape were analyzed by
using headspace sampling combined with GC-MS method．The results showed that garlic scape contains
23 kinds of volatile components，including 15 kinds of sulfur-containing compounds with 99.4% re lative
content．The 6 main components are diallyl disulfide（ 66.52%），1，3-dithiane（15.44%），diallyl
sulfide（7.15%），dimethyl disulfide（1.24%），diallyl sulfide（1.09%） and 2-allyl methyl sulfide
（2.66%）．Compared with steam distillation method，sulfur compounds detected by headspace sampling
method were fewer in kinds， but their contents were relatively higher，and the 3 main types ingredients：
sulfide，multi-dithiane and thiocyclohexylene could be detected.
2013 Vol. 1 (10): 80-83 [Abstract] ( 938 ) [HTML 1KB] [ PDF 996KB] ( 861 )
84 Molecular Detection of Pepper Viruses in Southern Vegetable Production
Bases
YAO Yu-Rong, CHEN Guo-Hua, FENG Lan-Xiang, XIE Bing-Yan
Pepper virus disease is a main disease in the southern vegetable production base in
Guangdong and Hainan Provinces．It is necessary to detect the kinds of pathogeny，so as to provide
references for the prevention of virus diseases. In this study，we conducted molecular detection with
RT-PCR method on the samples of pepper virus diseases or suspected virus diseases from these 2
provinces．The results showed that the detection rate of viruses was 55.67% in Guangdong Province. The
main viruses were CMV（ Cucumber mosaic virus） and PMMoV（ Pepper mild mottle virus）．Besides，
there were a few PVY（ Potato virus Y），PVX（ Potato virus X），and TYLCV（ Tomato yellow leaf curl
virus） detected．In Hainan Province，virus detection rate was 44.67%．CMV and PMMoV were the
major viruses．There was also a small quantity of PVY. In the fields，the complex infection of CMV and
PMMoV was the crucial problem in pepper production in Guangdong and Hainan Provinces.
2013 Vol. 1 (10): 84-89 [Abstract] ( 868 ) [HTML 1KB] [ PDF 970KB] ( 1210 )
90 Physiological Race Identification of Powdery Mildew for Edible Seed
Watermelon and Melon in Some Regions of Xinjiang
SU Rui, WANG Hui-Lin, GAO Qiang, JIA Song-Nan, ZHENG Jian
Ten pathogen collections of powdery mildew，which came from different grown
regions of Southern and Northern Xinjiang，were detected by standard system for identification of
powdery mildew. According to the internationally recognized melon differential standards for identifying
physiological races of powdery mildew，we confirmed that the disease was caused by Sphaerotheca fuliginea．
There were two races, race 1 and race 5．Race 1 was the dominant one in Northern Xinjiang，
and race 5 was in 2 regions of Southern Xinjiang.
2013 Vol. 1 (10): 90-94 [Abstract] ( 840 ) [HTML 1KB] [ PDF 875KB] ( 550 )
95 A New Cucumber F1 Hybrid for Greenhouse —‘Zhongnong No. 31’
GU Xing-Fang, ZHANG Sheng-Ping, WANG Ye, MIAO Han, ZHENG Qi-Gong
‘Zhongnong No.31’ is a newly bred cucumber F1 hybrid using inbred line 09676 as
maternal parent and 081006 as paternal parent．Its fruit is about 35 cm in length with short neck and
shining dark green skin．There are many white spines and small tuberculates on its fruit skin．It has
multi-resistances for downy mildew，powdery mildew and Fusarium wilt，et al．Moreover，it is
tolerant to low temperature and weak light. It is of early maturity and can sustainably bear fruit．It can
yield over 150 t·hm -2．It is suitable for greenhouse cultivation in northern China.
2013 Vol. 1 (10): 95-96 [Abstract] ( 627 ) [HTML 1KB] [ PDF 838KB] ( 603 )
97 A New Seedling-edible Chinese Cabbage F1 Hybrid —‘Sushengkuailyu’
LUO Zhi-Min, ZHANG Bin, WEN Feng-Ying, LIU Xiao-Hui, ZHAO Bing, WANG Chao-Nan, HUANG Zhi-Yin, LI Mei
‘Sushengkuailyu’ is a new seedling-edible Chinese cabbage F1 hybrid bred by crossing
self-incompatible line P42 with excellent DH line Feng520．It is upright and compact with 30 cm in
height．It has beautiful appearance with long obovate leaf，bright green and glossy leaves，flat foliar
without hairs，with green and wide flat petiole．Its leaf margin has few blunt lobes，with soft waxy
texture．It grows rapidly and can be harvested at 25 days when the number of blades is over 10 after
sowing．Its yield can reach about 50.475 t·hm-2．It is highly resistant to soft rot and virus disease，
and resistant to downy mildew．It is suitable to be planted all over China.
2013 Vol. 1 (10): 97-99 [Abstract] ( 680 ) [HTML 1KB] [ PDF 2585KB] ( 582 )
100 A New Thick-skin Melon F1 Hybrid —‘Yongtian No.7’
WANG Yu-Hong, ZANG Quan-Yu, MA erLei, DING Wei-Hong, HUANG Fu-Wei-Guo, HUANG Yun-Ping
‘Yongtian No.7’ is an F1 hybrid melon with crispy flesh and thick skin. It is bred by
crossing inbred line YW06-1 as female parent with YW10-3 as male parent．The fruit is ellipse in
shape．Its single weight is about 1.8 kg．The peel is white in color with dense net．Its flesh is orange in
color．It tastes crispy and fine．The Brix in the central part of fruit is over 15%．The fruit developing
period is about 40 days in spring and 38 days in summer．The whole developing period is about 100-
110 days in spring and 80 days in summer．Field investigation shows that‘ Yongtian No. 7’ is resistant
to gummy stem blight，and it can yield 16.2-36.0 t·hm-2．It is suitable for facility cultivation in spring
and fall in eastern China region.
2013 Vol. 1 (10): 100-102 [Abstract] ( 684 ) [HTML 1KB] [ PDF 921KB] ( 563 )
103 A New Bitter Gourd F1 Hybrid —‘Jiamei’
GAO Shan, LIN Bi-Ying, XU Duan-Xiang, LIN Feng
‘Jiamei’is a new bitter gourd F1 hybrid developed by crossing predominant female
line 43-C and inbred line 59-W．The plant grows vigorously. The first female flower is set at 8th-12th
nodes. The rate of female flowers is high and the fruit setting ability is strong．The fruit is of long rod
shape with 26-35 cm in length，5-7 cm in diameter，and 1.1 cm in flesh thickness．Its pericarp is
green and shiny．Its flesh tastes a little bitter. The single fruit weight is 400-500 g．It can yield about
45 t·hm-2．It is suitable to be cultivated in Fujian，Hubei and Sichuan Provinces.
2013 Vol. 1 (10): 103-105 [Abstract] ( 616 ) [HTML 1KB] [ PDF 911KB] ( 660 )
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