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| Initial Studies on Defense Gene Expression Mechanism of Phaseolus vulgaris L.
var. chinensis Hort. to Anthracnose at Seedling Stage |
| HU Guo-fu，XIAO Zi-heng，ZENG Ling-nan，ZHANG Ting，LI Cheng-yan |
| Life Science College， Northeast Agricultural University， Harbin 150030， Heilongjiang， China |
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Abstract Use susceptible cultivar‘Laolaishao’and resistant cultivar‘Dayoudou’as test
materials，the relative expression of 5 defense enzyme genes（chitinase， β-1，3-glucanase，SOD，
POD and LOX gene）and HRGP protein gene were detected and analyzed by real-time PCR after 2
cultivars infected by anthracnose in different times（ 0，3，6，9，12 d）. The results showed that in
addition to the β-1，3- glucanase gene and SOD gene，the other 4 genes expression were higher in
resistant varieties than the susceptible varieties before infected；after infected chitinase gene and the
LOX gene expression showed decreased-increased trend in susceptible cultivar，and resistant varieties
reached the peak，when susceptible varieties decreased to the minimum（3 d），the relative expression
of resistant varieties was higher than that of the susceptible ones in the whole stage（0-12 d）；β-1，
3-glucanase gene，POD gene and SOD gene were in the same trend，but the expression of resistant
varieties was higher than the susceptible varieties.
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Received: 09 October 2012
Published: 20 December 2012
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