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  Current Issue
 
2020 Vol.1 Issue.12,Published 2020-12-01
1
Hot!
2020 Vol. 1 (12): 1-6 [Abstract] ( 185 ) [HTML 1KB] [ PDF 1255KB] ( 913 )
7
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2020 Vol. 1 (12): 7-10 [Abstract] ( 235 ) [HTML 1KB] [ PDF 961KB] ( 571 )
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2020 Vol. 1 (12): 11-13 [Abstract] ( 166 ) [HTML 1KB] [ PDF 845KB] ( 441 )
14 Initial Analysis of Folic Acid and Its Derivative Contents in Different Tomato Species
CUI Yanan1，HAN Lida2，SHU Jinshuai1，WANG Xiaoxuan1，HUANG Zejun1，GUO Yanmei1，LIU Lei1，DU Yongchen1，ZHANG Chunyi2*，LI Junming1* Hot!
Adopting high-performance liquid chromatograph coupled with tandem mass spectrometry
（HPLC-MS/MS），this study conducted initial analysis on various forms of folic aid from different tomato
species and its derivative contents．The results showed that there were distinguished differences in total folic
acid contents in tomato fruits with different genotypes from cultispecies and wild species．The total folic acid
contents of tomato cultispecies was 1 532.79-4 530.72 μg · kg-1 and that in wild species was 2 587.85-4 035.93
μg · kg-1．The total folic acid contents of 17 Solanum pimpinellifolium accessions selected based on whole
genome sequence was 1 934.81-5 050.13 μg · kg-1．Thereinto，the folic acid contents of cultispecies M82 and
wild species LA0443 were higher，being 4 530.72 μg · kg-1 and 5 050.13 μg · kg-1，respectively．The results of
analyzing 12 kinds folic acid derivatives indicated that there were certain differences existing among folic acid
derivatives types from different germplasm resources，most of them containing at least 9 folic acid derivatives．
And the folic acid variation of these 17 S. pimpinellifolium accessions were more rich：5 accessions had 12
folic acid derivatives．While，the contents of tetrahydrofolate（THF），tetraglutamyl 5-formyltetrahydrofolate
（5-F-THFGlu4），folic acid（FA）and 10-formyl folic acid（10-F-FA） were lower or even undetectable in
some germplasm resources．In general，tomato was similar with the most crops， 5-methyl-tetrahydrofolate
（5-M-THF）was the major form of folic acid in fruit，followed by 5-formyltetrahydrofolate（5-F-THF）．
Moreover，a significant negative correlation was observed between 5-M-THF and 5-F-THF．
2020 Vol. 1 (12): 14-21 [Abstract] ( 263 ) [HTML 1KB] [ PDF 1226KB] ( 712 )
22 Establishment and Application of a Quantitative RT-PCR Detection System for Potato virus Y
LI Yuqi1，LI Qiuli1，WANG Yali1，HUANG Shanshan1，HU Chao1，XIAO Wanlu1，HUANG Yamin1，ZHAO Pengfei1，HUANG Wei2* Hot!
According to literature report and the design of primers registered by GenBank，a quantitative
RT-PCR（RT-qPCR）detection technology system for potato virus Y（PVY）was established．There existed
a favorable linear correlation（R2 = 0.991 2）between this technical standard curve of cycle threshold and
template concentration．This system could be used to detect PVY specifically，and the sensitivity was 1 000
times of the conventional RT-PCR and yet with good repeatability．PVY in potato plants from Shandong，
Qinghai，Guizhou，Heilongjiang，and Hebei 5 provinces，were detected by the established detection method．
The detection rates were 16.00，33.33，25.00，14.29 and 20.00 percentage point higher than that of the
conventional RT-PCR technology，respectively．Therefore，the established PVY RT-qPCR detection system
had the characteristics of strong specificity，high sensitivity，quick and accurate detection of PVY contents
from diseased potato seeds and plants．This study had provided effective technical means for early monitoring
and effective control of potato virus Y．
2020 Vol. 1 (12): 22-27 [Abstract] ( 181 ) [HTML 1KB] [ PDF 1323KB] ( 514 )
28 Small RNA Analysis and Resource Resistance Expression of Radish Virus Disease
GAN Caixia1，2，ZHANG Xingzhong1，CUI Lei1，2，YU Xiaoqing1，2，WANG Fei1，2，DAI Zhaoyi1，2，YAN Chenghuan1，2，DENG Xiaohui1，2*，YAO Minghua3，QIU Zhengming1，2，JIAO Chunhai3*
Virus disease is one of the main diseases harming radish．Plant virus disease detection
technology based on nucleic acid detection has been widely used owing to its wide detection range，high
sensitivity and suitability for mass detection．Adopting small RNA high-throughput sequencing method，this
experiment identified the kinds of radish virus disease，investigated and analyzed 264 accessions of radish core
germplasm resources and breeding lines resistance to virus diseases．Results indicated that the major pathogeny
of radish virus disease was turnip mosaic（TuMV）．Most of the tested core germplasm resources and breeding
lines expressed better resistance performance，and resistance between different breeding lines and sister lines
basicly verge to have no difference．Results of field investigation on resistance showed that there were 103
accessions with disease-resistance，of which 64 were high-resistant and 25 were immune．
2020 Vol. 1 (12): 28-34 [Abstract] ( 196 ) [HTML 1KB] [ PDF 1108KB] ( 386 )
35 Effect of Compound Functional Substrate on Growth and Fruit Quality of Melon
GONG Binbin1，WU Xiaolei1，WANG Shuo1，GAO Xiurui2，LIU Zhaoyang1，ZHANG Ying1，GAO Hongbo1*
Abstract：Taking melon variety ‘Xizhoumi No.25’as material，and peat，vermiculite，perlite
（volume ratio 2∶1∶1） as basic substrate，mixing 2 or 4 times Hoagland nutrient solution，water-retaining
agent and Bacillus subtilis to match substrates with different compound functions，this paper explored
the effects of nutrient solution concentration，water-retaining agent mixed with Bacillus subtilis on melon
growth，development and fruit quality．The results showed that comparing with the treament of only adding
2 and 4 times nutrient solution（T1，T5），the substrate mixed with nutrient solution，water-retaining
agent，and Bacillus subtilis（T4，T8） could significantly increase the biomass，leaf chlorophyll content，
photosynthesis，single fruit weight；promote the accumulation of soluble protein and sugar contents，and
reduce the content of nitrate and titratable acid of melon fruit．Results of multi-factor variance analysis showed
that water-retaining agent had the most evident effects on increasing leaf chlorophyll content，improving
water use efficiency，increasing single fruit weight and fruit soluble sugar content，and reducing nitrate
content．Bacillus subtilis had the most significant effect on increasing plant biomass，promoting
photosynthesis，enhancing fruit soluble protein content and reducing titratable acid content．Among all
treatments，T8 had the best result，i.e. mixing water-retaining agent with Bacillus subtilis and 4 times nutrient
solution．Therefore，in melon substrate cultivation，it is recommended to use peat，vermiculite and perlite
（volume ratio 2∶1∶1）as basic substrate，mixed with 4 times Hoagland nutrient solution，water-retaining
agent（8 mL · L-1） and Bacillus subtilis（2 g · L-1），naming compound functional substrate．
2020 Vol. 1 (12): 35-42 [Abstract] ( 259 ) [HTML 1KB] [ PDF 1019KB] ( 464 )
43 Effects of Different Manufacture Proposal of Planting-board and Drying Temperature on Seed Germination for Pakchoi
NIE Lei，XIE Qiang，ZHANG Jingjin，HUANG Danfeng
The planting-board is a kind of material that can produce vegetables by directly watering
after the seeds and cultivation medium are mixed，compressed and dried．This planting-board can be
coupled with water storage container to form a new system for vegetable cultivation on balcony．Taking the
pakchoi‘Xiawang’as material，this paper studied the effects of manufacture proposal of planting-board and
drying temperature on seed germination of pakchoi by orthogonal test and multi-index comprehensive grading
method．The results showed that the differences of comprehensive grading between different levels of waterretaining
agent and drying temperature were significant or extreme significant．The optimal manufacture
proposal for pakchoi planting-board was taking 4% volume of water-retaining agent，30% volume of
vermiculite，mixing them with matrix，and drying temperature at 45 ℃．
2020 Vol. 1 (12): 43-48 [Abstract] ( 160 ) [HTML 1KB] [ PDF 1051KB] ( 342 )
49 Comprehensive Evaluation and Selection of Identification Index for Low Nitrogen#br# Tolerant Varieties of Chinese Onion in Seedling Stage
LYU Lijun1，SHI Aokun1，YUAN Ruijiang2，LI Yansu1，YAN Yan1，YU Xianchang1，HE Chaoxing1*
Selecting 25 Chinese onion materials and adopting nutrient solutions with 2 nitrogen levels，
one of normal 14.0 mmol·L-1 and the other of mionectic 2.8 mmol·L-1，this experiment conducted hydroponic
treatment and comprehensive evaluation on low nitrogen tolerance of Chinese onion in seedling stage，
and screened out indicators for quickly evaluating low-nitrogen-tolerant Chinese onion in seedling stage，
hoping to provide references for identification of low-nitrogen-tolerant Chinese onions in seedling stage．
The results showed that there were different differences in 25 Chinese onion materials’ responses to low
nitrogen environment．According to the abilities of low nitrogen resistance，the test materials were divided
into 3 categories．Seven varieties of ‘Tianmingyiben’，‘Lucongza No.1’，‘Biancong’，‘Jicong No.1’，
‘Jiujingdacong’，‘Ribendongyue’ and ‘Shaocong’ were tolerant to low nitrogen．Eight varieties including
‘Liaocong No. 8’，‘Baoguanyuanzang’，‘Yuanzang’，‘Jicong No. 2’，‘Jucongwang’，‘Xincong No. 2’，
‘Zhangqiudawutong’ and‘ Qingye No. 1’ were moderate low-nitrogen-tolerant varieties.‘ Zhangqiutiegan’，
‘Hancong No. 1’，‘Qingza No. 2’，‘Lucongza No. 5’，‘Liaocong 16-12’，‘Tieganwutongwang’，
‘Lingbaidacong’，‘Hanfengjucong 90 bai’，‘Changyiqiubao’ and ‘Dugenjucong’，etc. 10 varieties were
low nitrogen sensitive varieties．The screened out relative shoot dry weight，relative root-shoot ratio，relative
root fresh weight，relative root length，relative stem diameter，and relative shoot nitrogen content could be
indicators for evaluating low-nitrogen-tolerance of Chinese onion in seedling stage．
2020 Vol. 1 (12): 49-55 [Abstract] ( 179 ) [HTML 1KB] [ PDF 1095KB] ( 577 )
56 Effect of Nutrient Concentration on Yield and Quality of Water Celery
LI Zhengpu1，2，TONG Jing2，3，WANG Liping1，JI Yanhai2，3，WANG Baoju2，3，WU Zhanhui2，3，WANG Suna1*
Concentration of nutrient solution directly affects the yield and quality of hydroponics
vegetables．This experiment studied the effects of different concentrations of nutrient solution on the growth
quality and yield of water celery，so as to screen out suitable nutrient solution concentration for the growth
of water celery in greenhouse．The results showed that the water celery biomass，chlorophyll content，
photosynthetic parameters，total flavonoids，VC，soluble protein content，etc. indexes firstly increased and
then decreased along with the increase of nutrient solution concentration．Thereinto，1.5 times concentration of
nutrient solution was the best，which can improve the overall quality and yield of water celery．
2020 Vol. 1 (12): 56-60 [Abstract] ( 147 ) [HTML 1KB] [ PDF 963KB] ( 465 )
61 Studies on Comprehensive Evaluation of Optimal Harvest Time of Brassica campestris L. ssp. Chinese Baby Greens Based on Nutrient Accumulation Dynamic
CHEN Ziyi，MO Kexun，ZHAO Shuo，ZHANG Jingjin*，HUANG Danfeng*
Abstract：The harvesting time of baby greens of pakchoi（Brassica campestris L. ssp. Chinese）is mainly
determined by traditional economic yield and its quality was neglected．Taking pakchoi variety ‘Huawang’
as test material，this experiment designed 4 plug cell sizes，including 72-cell，128-cell，200-cell，and 288-
cell，and conducted sampling 5，8，11，14，17 days after sowing（DAS）．Then，the variation rule of
physiological morphology and nutritional quality of the baby greens cultured in plug cells with different sizes
were studied．Principal component analysis was used to screen out the important indicators for comprehensive
quality of the baby greens．“Harvest index”was put forward to evaluate the comprehensive quality of baby
greens，hoping on the premise of guaranteeing the yield to determine the best harvesting time by nutritional
quality．The result indicated that the morphological variation trend of baby greens under different plug tray
were better matched with quadratic polynomial function．The VC content presented increasing trend along
with the time extending after sowing．The soluble protein content reached the maximum value on 14 DAS．In
earlier stage the nitrate content was high，then increased slightly after a significant reduction．The contents of
total chlorophyll and carotenoid under 72-cell，128-cell and 200-cell plug tray increased to the maximum value
on 14 DAS．While，those under 288-cell plug tray had no big changes on 5-17 DAS．Principal component
analysis extracted 4 principal components，and the cumulative variances contribution rate reached 72.244%．
The value of harvest index of baby greens under all sizes of plug trays reached maximum value on 14 DAS．The
value order from big to small was 288-cell ＞ 200-cell ＞ 128-cell ＞ 72-cell．The harvest indexes of 288-cell，
200-cell and 72-cell all decreased substantially until 17 DAS．While，the harvest index of 128-cell decrease
with smaller margin．Therefore，during the 5 harvesting stages investigated in this study，the best harvesting
period for the production of baby greens in glass greenhouse in spring in Shanghai region was 14 DAS，among
them the harvesting time of 288-cell and 200-cell plug trays was not suitable to delay．While，the 128-cell plug
tray could be properly delayed to 17 DAS．
2020 Vol. 1 (12): 61-70 [Abstract] ( 190 ) [HTML 1KB] [ PDF 1417KB] ( 337 )
71 Analysis of Phenotypic and Photosynthetic Characteristics of Common Bean Yellowing Mutant ylm
CHEN Qifu2，LIU Chang1，YANG Xiaoxu1，LIU Dajun1，YAN Zhishan1，FAN Youjun1，FENGCHEN Qifu2，LIU Chang1，YANG Xiaoxu1，LIU Dajun1，YAN Zhishan1，FAN Youjun1，FENG Guojun1*
The common bean mutant ylm is a yellowing mutant obtained by 60Co-γ-ray mutagenesis of
high-generation inbred line‘A18-1’，and its leaf color changes with the development process．This paper
conducted analysis on ylm leaf color change，photosynthetic pigment content，photosynthetic characteristics
of different developmental stages，and mutant agronomic traits at seedling stage and green ripening stage．
The results showed that the single plant weight，leaf area，stem thickness and main root length of mutant ylm
in seedling stage were all lower than those of the wild type．The dry and fresh weight of single plant in green
maturing stage，number of pods per plant，yield of fresh pods，number of seeds per pod，and pod length were
also significantly reduced．The total chlorophyll content and photosynthetic rate of the mutant increased along
with the darkening of leaf color．The reduction of photosynthetic pigment content inhibited the photosynthetic
rate，therefore affected the development of leaves，and also leading to the changes in stomatal conductance
and transpiration rate of leaves．
2020 Vol. 1 (12): 71-77 [Abstract] ( 164 ) [HTML 1KB] [ PDF 1865KB] ( 422 )
78 Studies on Measuring Methods of Leaf Area in Lettuce
LIU Xiaorui1，HUANG Chengdong1*，ZHU Hongwei2
Leaf area is an important factor for determining the amount of photosynthesis products in
plants．In order to obtain a rapid，accurate and flexible method for measuring vegetable leaf areas，this paper
took lettuce as an example and measured the plant leaf areas using Image J processing software，then carried out
comparative verification on the results of traditional testing method check counting method and paper weighing
method．The results showed that there was no significant difference between these 3 methods．By equation
linear regression analysis，we discovered that the correlation coefficients between these 3 methods were all
above 0.99．Therefore，the Image J processing software could be accurately applied to measure leaf area of
lettuce．
2020 Vol. 1 (12): 78-81 [Abstract] ( 194 ) [HTML 1KB] [ PDF 1180KB] ( 445 )
82 Analysis on Disciplinary Situation in Cruciferae Breeding from Global Sphere Based on Bibliometrics
JI Xuejing，KOU Yuantao，YE Sa，ZHU Liang*
Taking SCIE（Science Citation Index Expanded）and CPCI（Conference Proceedings Citation
Index）in the core collection of Web of Science as data sources，this study adopted the method of bibliometrics
to analyze the literature publication trends，funding sources，and high publication institutions，etc. in the
field of Cruciferae breeding from 1995 to 2018 in the global sphere．The results showed that 1995-2018，the
numbers of paper in the field of Cruciferae breeding were steadily increasing，with China，the United States
and Germany in the leading position．The global Cruciferae breeding literature mainly concentrated on plant
science，agronomy，horticulture，gene and genetics，etc. subjects．The National Natural Science Foundation
of China（NSCF）and China National Key Research and Development Program were the first and second
sources funding the literature projects，indicating that China national-level funding projects had given strong
support to research activities in cruciferae breeding field，and promoted the output of literature in this field．
2020 Vol. 1 (12): 82-88 [Abstract] ( 145 ) [HTML 1KB] [ PDF 2038KB] ( 380 )
89 A New Hot Pepper F1 Hybrid —‘Zhujiao 22’
LIANG Baoping，ZHAO Hongxing*，JIANG Jun，WANG Yong，LI Yan
‘Zhujiao 22’is a new hot pepper F1 hybrid developed by crossing male sterile two-type
line‘ABZhu0312’as female parent and inbred line‘Zhu0416’as male parent．It is of mid-early maturity．
Its growth period is about 163 days．The fruit is of cow-horn shape with tiny crinkle peel．The young fruit is
green in color，and tastes mild spicy．The fruit is 17.9 cm in length and 5.2 cm in width．Its pulp is 0.31 cm in
thickness．The average single fruit weight is 75.9 g．Its VC content in fresh fruit is 806 mg · kg-1．Each single
plant can bear 17.8 fruits．It can yield about 45 t · hm-2．In fields its resistance to virus disease，anthrax and
epidemic disease is stronger than that of the contrast‘Kangda301’．It is suitable for open field cultivation in
early spring in various regions of Henan Province．
2020 Vol. 1 (12): 89-91 [Abstract] ( 171 ) [HTML 1KB] [ PDF 2686KB] ( 300 )
92 A New Hot Pepper F1 Hybrid —‘Tianjiao No.21’
TANG Ruiyong，CHENG Fenglin，GAO Chenfa，ZHANG Xiaoying，WANG Lijun
‘Tianjiao No.21’is a new hot pepper F1 hybrid of fresh and dry dual-purpose type，developed
by crossing inbred line‘208-1-2-2-1-1-1’as female parent and inbred line‘113-2-3-2-1-1-1’as male
parent．It is of mid-early maturity．The plant growth is medium．The plant is 78.8 cm in height and
73.2 cm in spreading．The fruit is of line shape．Its fruit is 27.1 cm in length and 1.98 cm in width．
The pulp thickness is 0.23 cm．The single fruit weight is 29.15 g．The young fruit is green in color．
The matured one is red in color．Its VC content is 1 130 mg · kg-1，and capspaicin content is 42.9 mg ·
kg-1．It is mid-resistant to CMV，epidemic disease and anthrax．The fresh pepper yield is about 36 t ·
hm-2．It is suitable for planting in open fields in Gansu Province and regions with similar climate conditions．
2020 Vol. 1 (12): 92-94 [Abstract] ( 170 ) [HTML 1KB] [ PDF 2699KB] ( 419 )
95 A New Seed-using Squash F1 Hybrid —‘Jinghuzifeng No.1’
LI Haizhen，ZHANG Fan，ZHANG Guoyu，JIA Zhangcai，TIAN Jiaxing，XIE Shui
‘Jinghuzifeng No.1’is a new seed-using squash F1 hybrid bred by crossing inbred
line‘11552’as female parent and inbred line‘14600’as male parent．It is of short shoot type．It has high
yield and is highly resistant to diseases．The variety has strong growth vigor．Its growth period is about 100
days．The matured fruit is beige in color and of long stick shape．Its peel is thicker and hard to rot．Each
single fruit can bear about 320 seeds．The thousand kernel weight can reach 210 g．Its grains are full and
uniform in size．The seed is 1.92 cm in length and 1.01 cm in width．The grains have even size and green color
pulp．The protein content is 34.28%，dry matter content is 94.87%，fat content is 53.48%．The commodity
is good．Its plant is hard to premature senility．This variety is medium resistant to powdery mildew，ZYMV
and WMV．It can yield about 2.4 t · hm-2．It is suitable for open field cultivation in early spring in Inner
Mongolia，Xinjiang regions．
2020 Vol. 1 (12): 95-98 [Abstract] ( 281 ) [HTML 1KB] [ PDF 2756KB] ( 262 )
98 A New Broccoli F1 Hybrid —‘Bilyu 258’
DING Yunhua1，SONG Shuhui1，ZHAO Xuezhi1，HE Hongju1，HUANG Rong2，HU Jiankun2，HUANG Ruirong2*
‘Bilyu 258’is a broccoli F1 hybrid developed by crossing cytoplasmic male sterile
line‘CMS401’as female parent and inbred line‘202’as male parent．It is of mid-maturity．It takes about
85 days from transplanting to harvest．The plant is of erect type．Its leaf blades are slightly wrinkled with
margin wavy and thick wax on leaf surface．Its head is semi-circular，compact with small and green buds，not
turnning violet even under low temperature．The single head weight is about 0.5 kg．Its VC content is 1 061
mg · kg-1（FW）and protein content is 3.32%．It is rich in mineral nutrients．Its resistance to TuMV and
black rot in fields is equivalent both to the contrasts‘Youxiu’and‘Naihanyouxiu’．It can yield about 19.5 t ·
hm-2．It is suitable for autumn cultivation in Beijing，Hebei，Tianjin and Henan．
2020 Vol. 1 (12): 98-101 [Abstract] ( 151 ) [HTML 1KB] [ PDF 2765KB] ( 421 )
102
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2020 Vol. 1 (12): 102-103 [Abstract] ( 244 ) [HTML 1KB] [ PDF 961KB] ( 432 )
104
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2020 Vol. 1 (12): 104-106 [Abstract] ( 205 ) [HTML 1KB] [ PDF 1387KB] ( 590 )
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2020 Vol. 1 (12): 107-109 [Abstract] ( 168 ) [HTML 1KB] [ PDF 1399KB] ( 337 )
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2020 Vol. 1 (12): 110-112 [Abstract] ( 166 ) [HTML 1KB] [ PDF 1647KB] ( 448 )
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2020 Vol. 1 (12): 113-116 [Abstract] ( 154 ) [HTML 1KB] [ PDF 1572KB] ( 354 )
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2020 Vol. 1 (12): 117-122 [Abstract] ( 138 ) [HTML 1KB] [ PDF 1224KB] ( 303 )
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