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2012 Vol.1 Issue.20,Published 2012-10-15
1 An Observe of“Anther Culture in Pepper（Capsicum annuum L.）in vitro”
LI Su-wen，HUANG Ya-jie，XIAO Yu，ZHANG Bin*
On the basis of reading the existing relative documents about pepper（Capsicum annuum L.）in vitro anther culture，this paper summarizes and comments a newly published article“Anther Culture in Pepper（Capsicum annuum L.）in vitro”on the Acta Physiol Plant in 2011 from the effects of several main factors on pepper anther culture，plant regeneration ploidy identification and plant chromosome doubling ect．The authors think this article has briefly introduced the present status of pepper in vitro anther culture abroad，and the existing problems，which is of certain reference value．
2012 Vol. 1 (20): 1-6 [Abstract] ( 1249 ) [HTML 1KB] [ PDF 304KB] ( 796 )
7 Present Situation of Studies on miRNA of Plant under Adversity Stresses Using High-throughput Sequencing
YANG Jing1，2，QIN Zhi-wei1*
MicroRNA are 20-30 nucleotides long non-coding RNAs．They have played very important roles in plant growth and development，and also the process of adapting to different environmental stresses．In plant，there are big amount and many species of small RNAs．The emerge of high-throughput sequencing technology has significantly accelerated their discoveries．The authors summarize the situation about studying miRNA by using high-throughput sequencing technology under stress，so as to report the new discovery，function and evolution of miRNA in plants.
2012 Vol. 1 (20): 7-14 [Abstract] ( 1114 ) [HTML 1KB] [ PDF 329KB] ( 1388 )
15 Research Progress on Using Gene of Tomato yellow leaf curl virus（TYLCV）to Engineer TYLCV Resistance
LI Yun-zhou，LIANG Yan*，LI Cui，WANG Ling-hui
Tomato yellow leaf curl virus（TYLCV）has been spread all over the world and were main limiting factor for tomato production in major potato production areas of our country．The high variability among TYLCV makes the single gene resistance developed by conventional breeding can not last long．This paper summarizes internal and external studies on using  TYLCV gene-derived resistance（V1，C1，etc.）and analyzes why it can produce broad resistance．
2012 Vol. 1 (20): 15-19 [Abstract] ( 1346 ) [HTML 1KB] [ PDF 247KB] ( 1533 )
20 Analysis of International Competitiveness of China’s Vegetables Export Trade Based on“10+3”FTA Framework
DONG Ying，MU Yue-ying*
The integration of the East Asia region and its regional liberalization are constantly influencing the development of China’s vegetable export trade，and make the analysis of China’s international competitiveness of vegetable export extremely significant．On analyzing the basic characteristics of China’s vegetable export trade，this paper used price comparison within the region，the regional revealed comparative advantages and trade competitiveness index to carry out preliminary analysis．Finally the CMS model is applied to analyze the structural reasons for the changes in comparativeness．The results show that our vegetable export share to Japan and South Korea is declining，while that to ASEAN is continuously rising，but within the international and“10+3”range，the revealed comparative advantage of our vegetable export is weakening．Under“10+3”FTA framework，the demands for our vegetable export still show the trend of sustainable expansion．The export share and amount decline in mainly due to the improper structure of our vegetable export trade commodity．
2012 Vol. 1 (20): 20-26 [Abstract] ( 1049 ) [HTML 1KB] [ PDF 609KB] ( 804 )
27 Construction and Transformation of Vernalization-related Genes VIN3 Antisense Plant Expression Vector from Cabbage
YU Xi-hong，ZHAO Rong-qiu，JIANG Xin-mei*，LI Yan-ru
Taking plant expression vector pBI121 and BoVIN3-1 gene segment as bases，constructed vernalization-related gens VIN3 antisense plant expression vector from cabbage（Brassica oleracea var. captata L.），then inserted the BoVIN3-1 gene segment into XbaⅠand SmaⅠrestriction enzymes site between CaMV355 promoter and GUS gene in a reverted direction，and constructed plasmid pBI35S-BoVIN3-1．Through transferring it to cabbage flower bud by microinjection method，9 cabbages with Kan-resistance were obtained．The result of PCR amplifications proved that the obtained 5 cabbage were positive plant，and the positive plant rate was 55.6%．Compared the transgenic plant after vernalization treatment with the contrast，the transgenic plant vernalization response be delayed at certain extent．The result of RT-PCR amplifications proved that there was a little expression of VIN3 at small degree after low temperature treatment，and its transcription level increased with the prolongation of treatment time．The transcription reaches the peak at 50 days of vernalization.
2012 Vol. 1 (20): 27-35 [Abstract] ( 1261 ) [HTML 1KB] [ PDF 778KB] ( 935 )
33 Bacteria Diversity Analysis of Peat-moss for Culturing Vegetable Seedling
FANG Man-man，ZHANG Zhi-gang，DONG Chun-juan，SHANG Qing-mao*
In order to clarify the biological characteristics of peat moss from different areas，we compared and analyzed 8 bacterial diversities of KLASMANN（Germany），TERRACULT（Germany），PINDSTRUP（Denmark），KEKKILA（Finland），QINGYUAN（Liaoning），LIAOYUAN（Jilin），HUACHUAN（Heilongjiang）and YANSHOU（Heilongjiang）with PCR-DGGE method．The highest number of bands in LIAOYUAN，HUACHUAN and YANSHOU was 24，and the lowest number of bands in PINDSTRUP was 19．The differences between them were significant．The highest evenness index was YANSHOU，the lowest was KEKKILA．The differences between them were significant．The highest and lowest diversity indexes were QINGYUAN and KLASMANN，respectively．The highest similarity coefficient among the group was 72.9%，found between HUACHUAN and YANSHOU，while the lowest was 55.6%，found in KEKKILA and HUACHUAN．All these results indicated that there were differences in quantities and species of bacteria among peat-mosses from different areas．
2012 Vol. 1 (20): 33-38 [Abstract] ( 1888 ) [HTML 1KB] [ PDF 451KB] ( 805 )
39 Nitrate Content Discrepancies in Different Cultivars of Flowering Chinese Cabbage
SONG Shi-wei，YI Ling-yan，LIU Hou-cheng，SUN Guang-wen，CHEN Ri-yuan*
Biomass and nitrate content in 41 cultivars of flowering Chinese cabbage（Brassica campestris L. var.?utilis Tsen et Lee）were studied under hydroponic conditions．The results showed that the biomass and maturity stage of flowering Chinese cabbage had significant positive correlation．Cultivars of late maturity acquired large biomass，and cultivars of early maturity acquired smaller biomass．There were significant differences in nitrate contents of different flowering Chinese cabbage cultivars，and the nitrate content in flower stalk was obviousely higher than that in leaf blades．41 cultivars of flowering Chinese cabbage were divided into 3 groups of high，medium and low nitrate contents according to cluster analysis．The group with high nitrate content（11 cultivars）had lower biomass，and the group with low nitrate content（18 cultivars）had higher biomass，while the group with medium nitrate content（12 cultivars）had medium biomass．
2012 Vol. 1 (20): 39-43 [Abstract] ( 1058 ) [HTML 1KB] [ PDF 305KB] ( 777 )
44 Establishment of High-frequency Regeneration System from Cotyledons with Petiole in DH Lines of Broccoli
SHENG Xiao-guang，GU Hong-hui*，YU Hui-fang，WANG Jian-sheng，ZHAO Zhen-qing
The high frequency of shoot regeneration from cotyledons with petiole in broccoli（Brassica oleracea L.?var.?italica Plenck）was studied and the effect of the different genotypes，different mass concentration plant growth regulator，different age of explants and different concentration AgNO3 on regeneration frequency of adventitious buds were studied．The results showed that 2 DH lines，3-6 and 3-7，obtained high shoot differentiation rate（96.9% and 97.8%，respectively），after the explants were pre-cultured for 8 days，then placed in the MS medium supplemented with 0.02 mg·L-1 NAA，1.0 mg·L-1 6-BA，4 mg·L-1 AgNO3，10 g·L-1 agar and 30 g·L-1 sucrose．The frequence of shoot regeneration was 15 and 14．The feasible rooting medium was MS + 0.05 mg·L-1 NAA +7 g·L-1 agar + 30 g·L-1 sucrose and the rooting rate reached to 100%．
2012 Vol. 1 (20): 44-48 [Abstract] ( 939 ) [HTML 1KB] [ PDF 402KB] ( 518 )
49 Effects of Different Nutrition Solution Concentrations on Cucumber Seedling Growth under Seedling Culture without Substrate Nutrient Solution
REN Rui-zhen1，WU Zhan-hui2，CHEN Hai-li2，YU Ping-bin2，WU Zhen1，LIU Ming-chi2*
By seedling culture without substrate nutrient solution，this experiment studies the effects of different nutrition solution concentrations on the growth of cucumber（Cucumis sativus L.）seedlings. The results showed that the seedling had lower plant height，thinner stem diameter，lower biomass，weaker resistance to stress，smaller root volume and lower seedling index under lower concentration treatments（0.5 and 1.0 times nutrition solution），which were related to deficient nutrition supply under low concentrations，thus the growth and development of seedlings were slowed down．Under higher concentration（2.0 times nutrition solution）treatment，the seedling growth was inhibited during the earlier stage．While in the late stage，although the seedling growth speed was accelerated，its robust degree was still low，due to the un-eliminated stress．The treatment of 1.5 times nutrition solution had significantly promoted the seedling growth，effectively improved the seedling biomass，increased robust seedling index and root activity，and had better coordination between seedling growth rate and resistance．Its promotion role was much bigger than stress effect．Therefore，1.5 fold of nutrition solution is the optimal concentration for nutrient solution seedling culture without substrate．
2012 Vol. 1 (20): 49-55 [Abstract] ( 1233 ) [HTML 1KB] [ PDF 581KB] ( 871 )
56 Effects of Salt Stress on Sugar Content and Sucrose Metabolism in Tomato Fruit
LU Shao-wei1，QI Fei1，LI Tian-lai2*
Tomato（Lycopersicon esculentum Mill.）cultivar‘Liaoyuanduoli’was used，salt stress〔50 mmol·L-1 NaCl and iso-osmotic NaNO3、KCl、KNO3、CaCl2 and Ca（NO3）2〕treatments were conducted in the fruit development period（from 20 days after flowering to fruit ripening）to study the effects of different types of salt stress on sugar content and sucrose metabolism in tomato fruit．The results showed that the three Cl- salt treatments had increased the contents of soluble solids，soluble sugar，organic acids and sugar-acid ratio in matured tomato fruit．Further analysis indicated that salt stress treatments had enhanced hexose（fructose + glucose）content in various parts of tomato fruit，strengthened sucrose invertase（acid invertase + neutral invertase）activity．Under various treatments，the changes in contents of soluble sugars and hexose had converted to direct proportion with the changes in sucrose invertase activity．Thus，under salt stress the increase of soluble sugar content in tomato fruit was due to the strengthening of sucrose invertase activity．The best salt for improving tomato quality is KCl．
2012 Vol. 1 (20): 56-61 [Abstract] ( 1458 ) [HTML 1KB] [ PDF 565KB] ( 1074 )
62 Effects of Different Control Measures on Cucumber Bleeding Sap and Its Nutrient Contents in Greenhouse
XU Yu，JIANG Li-hua，ZHENG Fu-li，LIN Hai-tao，SONG Xiao-zong，TAN De-shui，GAO Xin-hao，LIU Zhao-hui*
A field experiment was conducted to study the effects of different control measures on cucumber（Cucumis sativus L.）yield，bleeding sap and its nutrient content in greenhouse．The results showed that the root bleeding intensity on day was higher than that in night．And with the prolongation of growing period，the bleeding intensity and amount showed a increasing trend．But under the influence of root growth and external environment，the increased degree became smaller at the later stage．There was many free amino acid in bleeding sap，but the main nitrogen form was nitrate N．Compared with the fertilization model used by peasant，the bleeding amount and the contents of nitrate N，ammonium N，free amino acid and K in the bleeding sap could increase significantly through straw returning．This measure could also increase the root activity and plant absorption of N and K．The yield slightly increased about 1%．For the influence of irrigation frequency，the above indicators of bleeding sap under drip irrigation measure were all decreased significant and the yield decreased 13.1% with significant differences．
2012 Vol. 1 (20): 62-67 [Abstract] ( 1174 ) [HTML 1KB] [ PDF 328KB] ( 651 )
68 Effects of Slow Release Compound Fertilizer on Yield，Quality and Nutrient Content of Tumorous Stem Mustard
WANG Fei，WANG Zheng-yin*，ZHAO Huan，ZHANG Guo-ping，L Hui-feng
Field experiments were conducted to study the effects of different slow release compound fertilizers on biological traits，yield，quality and nutrient content of tumorous stem mustard（Brassica juncea Coss.?var.?tumida Tsen et Lee）．The results showed that compared with special fertilization，application of slow release compound fertilizers was effective to improve the biological traits and yield of tumorous stem mustard．The treatment of slow released compound fertilizer dropped the contents of soluble sugar and increased free amino acids of tumorous stem，but the change of vitamin C was not outstanding，which also decreased the content of nitrate．On the whole，slow released compound fertilizer could increase the total nitrogen，phosphorus and potassium contents of stem and leaf of tumorous stem mustard．Considering various factors，the treatment of reducing 5% slow release compound fertilizer application（1 225.5 kg·hm-2）is an optimum choice.
2012 Vol. 1 (20): 68-72 [Abstract] ( 2292 ) [HTML 1KB] [ PDF 331KB] ( 667 )
73 Dynamic Change of Ginger Rhizosphere Soil Enzyme Activities and Microorganism Quantity under Different Fertilizers Combined Application
KONG Xiang-bo
Taking ginger（Zingiber officinale Rosc.）as experiment material，this paper analyzed the effects of different fertilizers combined application on ginger root system and rhizosphere soil enzyme activities and micro-organism quantity．The results show that the biological organic fertilizer can improve the fresh weight of ginger root and strengthen the root activity．The fresh weight of ginger root was higher than that applying common chemical fertilizer or common organic fertilizer by 28.3% and 16.4%，and the root activities were increased by 41.2% and 20.6%．The biological organic fertilizer can increase the soil organic matter．The max value of soil organic matter is achieved during vigorous stage. The contents were higher than that applying common chemical fertilizer and common organic fertilizer by 45.4% and 11.1%，respectively．This fertilizer could significantly improve soil microbe quantity．Compared with that applying common chemical fertilizer，the population of bacteria increased by 449.1%，that of fungi increased by 374.3%，that of actinomycetes increased by 489.8%，and the total population of these 3 kinds of microorganism was increased by 451.5%．Its increased quantity was higher than that applying common organic fertilizer．The activities of invertase，urease and catalase in soil were increased obviously by applying biological organic fertilizer．Thus the decomposition and transformation of soil organic matter was promoted.
2012 Vol. 1 (20): 73-77 [Abstract] ( 1182 ) [HTML 1KB] [ PDF 440KB] ( 565 )
78 Effects of Different Trace Irrigation Pipe Depths on Eggplant Growth，Yield and Water Utilization Efficiency in Solar Greenhouse
YANG Ming-yu1，AN Shun-wei2，ZHOU Ji-hua2，SHI Wen-xue2，XIAO Chang-kun1，HUANG Hui-ling1
Taking‘Jingqie No.?1’as material，the effects of different trace irrigation pipe depths on irrigation quantities，water consumption，yield and fruit quality of spring eggplant（Solanum melongena L.）in solar greenhouse were studied．The results showed that different trace irrigation pipe depths could promote plant growth and increase yield．Compared with the surface casing treatment，when the trace irrigation pipe depth were 10 cm，the yield of eggplant increased by 14.7%．While，with the increasing of trace irrigation pipe depth，the irrigation quantities and water consumption reduced，but the water use efficiency of the trace irrigation pipe depth 10 cm treatment was highest，which reached 23.5%．Considering various factors，among all the trace irrigation pipe depths，the highest eggplant yield and water use efficiency and lower water consumption were appeared in 10 cm depth．Therefore，trace irrigation pipe depth 10 cm was the appropriate trace irrigation pipe depth for spring eggplant production in solar greenhouse．
2012 Vol. 1 (20): 78-82 [Abstract] ( 1145 ) [HTML 1KB] [ PDF 503KB] ( 704 )
83 Effects of Different Resistant Rootstocks on Yield and Quality of Grafted Tomato and Control Effects of Meloidogyne incognita
CHEN Zhen-de1，WANG Pei-sheng1，ZHOU Ying1，JI Yu-ling2，LIANG Peng1，3，WAN Zong-jun1，4，HAO Jun-jie1
Taking different tomato（Lycopersicon esculentum Mill.）stocks as materials，this experiment studies the effects of different graft stocks on tomato yield，quality and control effects on Meloidogyne incognita．The results indicated that after grafted to tomato，the control effects on Meloidogyne incognita of 6 stocks were all above 98.6%．Especially they were high resistant to root-knot nematode．Compared with own-rooted，the yields of tomato grafted with resistant rootstocks could increase by 15.4%-19.4%．Rootstock grafting had no influence on soluble protein，soluble solid，titrable acid content of tomato fruits，whereas the contents of soluble sugar，VC，lycopene and nitrate were mostly lower than the own-rooted contrast．Judging from the combined results，using‘Guozhen No.?1’and‘Telusi’as resistant rootstocks，the grated tomato plants have good controlling effect on southern root-knot nematode，and these 2 rootstocks have very little influence on tomato quality，but have higher yield potential.
2012 Vol. 1 (20): 83-87 [Abstract] ( 1451 ) [HTML 1KB] [ PDF 299KB] ( 800 )
88 Isolation and Identification of Garlic Dry Rot Disease Pathogen
LI Xu-shuang，CHEN Dian，LIANG Yu，WANG Yong*
In order to study the pathogens of garlic dry rot disease，this study used the susceptible garlic（Allium sativum L.）strain Z-1001 for pathogen isolation，single-spore purification and pathogenicity determination．The results revealed that the F strain and J strain were main garlic dry rot pathogenic strains．The results of pathogen morphology observation and rDNA ITS sequence analysis showed that F strain was Fusarium oxysporum Schlecht，while J strain was Fusarium proliferatum（Matsush.）Nirenberg，which in turn revealed that F.?oxysporum Schlecht and F.?proliferatum（Matsush.）Nirenberg were the main pathogens of garlic dry rot disease.
2012 Vol. 1 (20): 88-93 [Abstract] ( 1147 ) [HTML 1KB] [ PDF 752KB] ( 748 )
94 A New Mid-early Maturity Sweet Pepper F1 Hybrid—‘Zhongjiao No. 105’
WANG Li-hao，MAO Sheng-li，GUO Jia-zhen，ZHANG Zheng-hai，DU Mei-zhen，ZHANG Bao-xi*
‘Zhongjiao No.?105’is a new sweet pepper F1 hybrid using inbred line 78090-3-1 as female parent and 0516 as male parent．It is of mid-early maturity．The first floral bud is borne at node 9-10．The mature-green fruit harvest begins at 35 days after colonization．The matured fruits are 130- 150 g in weight，10 cm in length and 7 cm in diameter with light green color，smooth surface and 3 or 4 lobes．It is high tolerant to heat and cold，resistant to TMV，moderately resistant to CMV and blight．It has high and stable yield，and can keep better fruit setting rate and good commodity even during the middle and late reproductive period．The normal fruit yield can reach 45.0 t·hm-2．It is suitable for cultivation in open field and greenhouse.
2012 Vol. 1 (20): 94-96 [Abstract] ( 948 ) [HTML 1KB] [ PDF 169KB] ( 567 )
97 A New Eggplant F1 Hybrid—‘Zheqie No. 3’
MAO Wei-hai，BAO Chong-lai，HU Tian-hua，ZHU Qin-mei，DU Li-ming，HU Hai-jiao
‘Zheqie No.3’is a new eggplant F1 hybrid developed by crossing 2 inbred lines E673-10-2-1-5-1-1-2-1 as female parent and E22 as male parent．It is of early maturity．It grows vigorously and has high fruit setting rate．Its fruit is of long club and with purple skin．The fruit has shining gloss and good commodity．It is resistant to verticillium wilt and middle resistant to bacterial wilt．It yield is about 57.0 t·hm-2．It is suitable for cultivation in Zhejiang，southern Anhui and Jiangxi Province．
2012 Vol. 1 (20): 97-99 [Abstract] ( 1292 ) [HTML 1KB] [ PDF 283KB] ( 752 )
100 A New Cucumber of Southern China Type F1 Hybrid—‘Lüjian’
LIU Jian-hui，ZHANG Pei-zhi，LIU Si-yu
‘Lüjian’is a new cucumber F1 hybrid of southern China type bred by crossing Y-26-2 as female parent and Y-12-6 as male parent．It is of early maturity．Its melon is straight with good uniformity，22 cm in length，3.5 cm in diameter．It has white thorn，scarce thorn tumor．It is bright green in color and very glossy．It tastes slightly sweet and fragrant without bitter flavor．It is resistant to downy mildew and Fusavium wilt．It yields about 45.0 t·hm-2．It is suitable for cultivation in greenhouse in spring and open field in early spring.
2012 Vol. 1 (20): 100-101 [Abstract] ( 876 ) [HTML 1KB] [ PDF 234KB] ( 606 )
102 A New Fresh Sweet Corn F1 Hybrid—‘Tianxian No.2’
XIE Jun-xian，NIU Xiu-qun*
Tianxian No.2’is a single-cross hybrid sweet corn bred by crossing inbred-line I31 as female parent and inbred-line I21 as male parent．Its ear is cylindrical，21.6 cm in length．It has 14-16 rows in ear．The hundred fresh kernel weight per ear is 42.5 g．The kernels are multi-colored when harvested．The soluble sugar content is 13.45%．It has good quality and strong disease resistance．It takes 82 days from seeding to harvest．Its common yield is about 15.0 t·hm-2．It is suitable for cultivation in northern China，and can be test planted in other parts of China.
2012 Vol. 1 (20): 102-104 [Abstract] ( 888 ) [HTML 1KB] [ PDF 221KB] ( 534 )
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