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  Current Issue
 
2009 Vol.1 Issue.12,Published 2009-06-15

专论与综述
1 Survey of Potato Virus Detection Technique
MAO Yan-zhi
This paper summarized the progress made in the research about potato virus
detection.It briefly introduced the methods of visual observation,indicator plants,
electron microscopic method,serological detection and molecular biology,etc.It also
provided a brief evaluation on each method.
2009 Vol. 1 (12): 1-6 [Abstract] ( 1180 ) [HTML 0KB] [ PDF 382KB] ( 1324 )
试验研究
7 Effect of Post-harvest Washing with Different Disinfection and Temperature on Controlling Rotten Rates of Tomatoes during Shelf Life
WANG Jin-yu;ZHANG Xue-jie*;GUO Ke;SUN Ya-jun;Robert Holmes;Simone Kreidl

Effects of different washing treatments on tomato rotten rates within 7 days

shelf life,packed by PE film at ambient temperature(28-30 ℃),were investigated,including

tap water,100 mg·kg-1 NaClO and 5 mg·kg-1 ClO2 under different temperature(5 ℃ higher and

lower than fruits’ temperature,respectively).The results showed that the rotten rates of

the washed tomatoes were decreased significantly from 47 %(no washed,CK)to 9 %-26 %,and the

temperature has not taken effect on reducing rotten rate for tap water and ClO2

treatments.However,it reduced the rotten rate significantly by 100 mg·kg-1 NaClO washing

treatment with 5 ℃ higher than fruits temperature ompared with 5 ℃ lower than fruits’

temperature treatment,which reached 9 %,the best treatment after 7 days storage.

2009 Vol. 1 (12): 7-10 [Abstract] ( 1238 ) [HTML 0KB] [ PDF 279KB] ( 952 )
11 Comparison of Chemical Composition and Nutritional Components of Genuine Traditional Lycium barbarum L.Produced from Different Habitats
MA Jian-jun;ZHOU Tao;ZHU Li-xin*

It is a comparison about the chemical composition and nutritional components of

the fruit and leaf of genuine traditional Lycium barbarum L. ‘Ningqi No.1’produced from

Luhuatai Horticultural Farm of Yinchuan and Zhongning County.The results show that the

highest amino acids contents are Asp,Glu,Ala,Arg and Pro both in fruits and leaves,but no

significant difference was observed between the fruits produced in Luhuatai Horticultural

Farm of Yinchuan and Zhongning County.The lycine content of Air-drying fruit produced in

Zhongning County was 44.2 % higher than that produced in Luhuatai Horticultural Farm of

Yinchuan.The polysaccharide content of air-drying fruit and leaf produced in Luhuatai

Horticultural Farm of Yinchuan was higher than that produced in Zhongning County.The VC

content of air-drying fruit and leaf produced from Luhuatai Horticultural Farm of Yinchuan

was distinctively higher than that produced in Zhongning County.The loss of carotene

content of freeze-drying fruit was higher than that of air-drying fruit.While the loss of

VC content of air-drying fruit was higher than that of freeze-drying fruit.

2009 Vol. 1 (12): 11-14 [Abstract] ( 1216 ) [HTML 0KB] [ PDF 114KB] ( 1352 )
15 Effects of Molybdenum and Manganese on Nitrate Reductase Activity and Quality of Brassica campestris ssp.chinensis
CHEN Long-zheng;CHEN Jie;LIANG Liang;XU Hai;SONG Bo;SU Xiao-jun;YUAN Xi-han*

Effect of molybdenum and manganese on nitrate reductase activity,nitrate content

and nutrient quality in Brassica campestris ssp.chinensis were studied using solution

culture by spraying Mo and Mn elements on leaf,root and leaf combined with root

treatment.The results indicated that nitrate reductase activity was significantly improved

by Mo treatment,which caused significant reduction of nitrate content.Although,no

remarkable effect on improving nitrate reductase activity was found by Mn treatment,nitrate

content was also reduced.Both Mo and Mn could improve the contents of soluble sugar and

VC.Compared with single treatment of Mo or Mn,the reduction of nitrate content,improvement

of soluble sugar and VC contents were more distinct by treatment with Mo plus Mn.

2009 Vol. 1 (12): 15-18 [Abstract] ( 1302 ) [HTML 0KB] [ PDF 193KB] ( 873 )
19 Establishment and Optimization of SSR Detection System in Cabbage(Brassica oleracea var.capitata L.)
SI Jun;LI Cheng-qiong*;SONG Hong-yuan;REN Xue-song;SONG Ming;WANG Xiao-jia

In this study,it established and optimized SSR detection system including

reaction components,amplified program,by using cabbage inbred lines 15R(‘Nanbao’)and

14S(‘Beiheidapingtou’).A stable SSR-PCR system was followed:10.00 μL reaction solution

contained 1×Buffer,50.00 μmol·L-1 dNTPs,0.40 U Taq enzymes,0.40 μmol·L-1 SSR primers

pairs,1.00 μL DNA(60.00 ng·μL-1).PCR amplifying program was divided into two steps,after

one denaturing step of 5 min at 94 ℃,DNA was denatured for 45 s at 94 ℃,the annealing

temperature was 60 ℃ and dropped 0.5 ℃ for each cycle,until a final annealing temperature

of 50 ℃ was reached.For the last 15 cycles of the amplification,an annealing temperature

of 55 ℃ was employed.When all cycles were finished,amplification ended for 10 min at 72 ℃

.The polyacrylamide gel electrophoresis was used in the detection of PCR amplification.

2009 Vol. 1 (12): 19-23 [Abstract] ( 1200 ) [HTML 0KB] [ PDF 678KB] ( 890 )
24 Studies on Artificial Inoculation Method to Test Resistance to Pepper Leveillula taurica
WANG Xuan;WANG Li-hao;SHI Yan-xia;MAO Sheng-li;LI Bao-ju*;ZHANG Bao-xi*

Taking different disease resistance pepper varieties(lines)as material,this paper

studies on the artificial inoculation as identification method of Leveillula taurica to

pepper from inoculation concentration,investigation time,age of inoculated seedling,disease

occurring condition after inoculation plant inoculation treatment method,etc.The results

showed that the most effective artificial inoculation method was inoculating at 15-leaf

stage and sprayed by a suspension of 1×104 conidia per mL and kept in dark and under 100 %

humidity during first 24 h after inoculation and then in 23-27 ℃ and 60 %-85 % humidity in

daytime,16-18 ℃ and 95 %-100 % humidity at night.

2009 Vol. 1 (12): 24-27 [Abstract] ( 1499 ) [HTML 0KB] [ PDF 224KB] ( 918 )
28 Preliminary Report about Screening of Antagonistic Actinomycetes against Phytophthora capsici and Its Control Effect
YI Long;XIAO Chong-gang;ZHANG Ying-fang;WU Qiong

276 isolates were obtained from rhizospheric soils of pepper.It was found that 7

potential biocontrol actinomycetes showed good antagonistic effects on Phytophthora capsici

by pairing culture.The inhibition zone diameter ranged from 7.8 mm to 13.0 mm.The result

from the experiment trial at greenhouse showed that CQ21-3 strain could effectively control

the pepper phytophthora blight,the control efficiency reached 73.2 %.The result from

culture filtrate showed that CQ21-3 isolate was effective for inhibiting mycelium growth

and reducing spore germination of P.capsici in certain of concentration.

2009 Vol. 1 (12): 28-32 [Abstract] ( 1136 ) [HTML 0KB] [ PDF 374KB] ( 873 )
33 Insecticidal and Antibiotic Activities of Symbiotic Bacteria Strains to Laphygma exigua and Colletotrichum gloeosporiodies
WANG Huan;CONG Bin*;LIU Yan-qun;DONG Hui;SUI Xin

Forty strains of symbiotic bacteria were isolated from entomopathogenic

nematodes.Bioassay results showed that all strains had virulent activity to the first

instar larvae of Laphygma exigua and Colletotrichum gloeosporiodies to some extent,among

which Xenorhabdus nematophila A24-1 strain was the best one.The average corrected

mortalities to Laphygma exigua was 100.00 %,and the diameter of antibiotic circle to

Colletotrichum gloeosporiodies was 40.00 mm.

2009 Vol. 1 (12): 33-36 [Abstract] ( 1245 ) [HTML 0KB] [ PDF 113KB] ( 870 )
37 Allelopathy Regulation of Gingko Leaves on Autotoxicity of Pepper Plants’ Fermentation Broths
HOU Yong-xia;ZHOU Bao-li*;WU Xiao-ling;FU Ya-wen

By applying the method of water culture with 1/2 Hoagland solution,this paper

studied the autotoxicity of pepper plants’ fermentation broths and allelopathy regulation

on it by gingko leaves’ fermentation broths and decomposition solutions that the

fermentation broths were decomposed again after the leaves were removed.The results showed

that under the autotoxicity of pepper plants’ fermentation broths,their root vigors were

declined and the relative conductivity was aggrandized.Gingko leaves’ fermentation broths

enhanced the autotoxicity of pepper plants’ fermentation broths,and showed positive

correlation with its concentration.Gingko leaves’ decomposition solutions have alleviated

the autotoxicity of pepper plants’ fermentation broths.The alleviated effects depend on

the concentration of gingko leaves’ decomposition solution.

2009 Vol. 1 (12): 37-40 [Abstract] ( 1117 ) [HTML 0KB] [ PDF 423KB] ( 836 )
41 Initial Studies on Allelopathy of Liquid Extract of Allium ascalonicum L.on Seed Germination and Seedling Growth of Lactuca sativa L.,Brassica chinensis L.,Raphanus sativus L.,Cucumis sativus L.
HAN Xiao-yan;LUO Qing-xi*;LIU Hai-li;WANG Li-na;GAO Yang

A preliminary study was made on the allelopathy of liquid extract of Allium

ascalonicum L.to Lactuca sativa L.,Brassica chinensis L.,Raphanus sativus L.and Cucumis

sativus L.The results showed that the extract from Allium ascalonicum L.had some inhibit

effect on the germination of seed of Lactuca sativa L.and Raphanus sativus L.,had little

inhibit effect on the germination of seed of Brassica chinensis L.and Cucumis sativus L.The

results showed some stimulating effect on the growth of Lactuca sativa L.,Brassica

chinensis L.,Raphanus sativus L.and Cucumis sativus L.,as a whole.

2009 Vol. 1 (12): 41-44 [Abstract] ( 1172 ) [HTML 0KB] [ PDF 203KB] ( 736 )
45 Pilot Studies on Effects of Induction Resistance to Black Spot Disease in Post-harvest Pepper
GUO Hong-lian;WANG Bo-yu;LIU Xiao-yuan

Alternaria capsici-annui was studied in this article,which caused one of the main

decay of postharvest pepper.Oligochitosan and salicylie acid were used on pepper storage

and their effects to spore of A.capsici-annui were analyzed.The results showed that

Oligochitosan and salicylie acid could have significant effect on reducing black spot

incidence of post-harvest pepper,and the optimum concentration was 75 mg·L-1.

2009 Vol. 1 (12): 45-47 [Abstract] ( 1070 ) [HTML 0KB] [ PDF 236KB] ( 779 )
48 Studies on Breeding Method for Genetic Male Sterile Line of Chinese Cabage with Orange Head
  1. FENG Hui;JIANG Nan;LI Cheng-yu
The fertile plants MsfMs of Genetic Male Sterile AB Line‘AB01’of common Chinese cabbage and stably inherited inbred lines of Chinese cabbage with orange color‘Beijing-1’,‘Shandong-1’were used to prepare F1,F2 and BC1.Their head color and stamen fertility were appraised,the inheritance rule of head color and the relations between head color,flower color and fertility were analyzed,how the male sterile genes transferred to Chinese cabbage with orange head was explored.The results showed that head color was controlled by a pair of recessive allele,orange head and orange flower were closely linked or even controlled by the same gene,head color and fertility genes reacted independently,and their fertility genes were both msms.On this basis,it put forward a genetic model that it could use genetic male sterile line of common Chinese cabbage to transfer those with orange head.
2009 Vol. 1 (12): 48-52 [Abstract] ( 1156 ) [HTML 0KB] [ PDF 312KB] ( 791 )
53 Blur Comprehensive Evaluation of 11 Mini Watermelon Cultivars
HOU Pei;ZHANG Xian

The multiple traits of mini watermelon can be reflected by several specific factors.The test divided the

traits into three types which composed sets and subsets of estimation elements.11 mini watermelon materials were

analyzed by comprehensive blur evaluation.These 3 characters double-fuzzy comprehensive evaluation results:

‘Linglongwang’general ranking was the first,Hai 024×Hai 011 F1 was the second,and Hai 024×Hai 011 F1 can be used

as a similar species of‘Linglongwang’got further comparative experiment.

2009 Vol. 1 (12): 53-58 [Abstract] ( 1007 ) [HTML 0KB] [ PDF 328KB] ( 753 )
53 Studies on Bud Differentiation of Xinjiang Artemisia dracunculus L.
ZHAO Jun;SHA Yu-cang;YANG Chang-kai;MU Wan-fu;XIN Jian-hua*

The flower bud differentiation process of Xinjiang Artemisia dracunculus L. lasted for about one month from

late May to July.The process could be divided into three phases.They are floral axis differentiation

phase,inflorescence differentiation phase and flower organ differentiation phase.The apical flower bud and laterval

flower bud of tarragon can turn from vegetative cone and half flourish is imperfect flower and tublar floret is

perfect flower.

2009 Vol. 1 (12): 53-58 [Abstract] ( 987 ) [HTML 0KB] [ PDF 1059KB] ( 811 )
64 Effects of Mixing Peanut Chaff Substrate on the Growth and Quality of Tomato Plug Seedlings
YANG Hong-li;WANG Zi-chong;ZHANG Shen-pu;LIANG Xin-an;SUN Xin-zheng
The effect of seven mixing peanut chaff substrate treatments on tomato seedlings was studied in the paper.The results showed that the substrate’s composition of peanut chaff∶cattle manure∶slag= 6 V∶2 V∶2 V was an appropriate match.The 6 indexes of this treatment as:stem diameter,plant height,stem and leaf fresh weight,stem and leaf dry weight,root dry weight and strong seedling index were higher than that of the other 6 treatments and were significantly higher than that of the control(peat∶vermiculite=2 V ∶1 V).The leaf chlorophyll content was not significantly different with that of the control.
2009 Vol. 1 (12): 64-67 [Abstract] ( 1243 ) [HTML 0KB] [ PDF 265KB] ( 1032 )
品种选育
68 A New Seedling-edible Chinese Cabbage F1 Hybrid —‘Jingyankuaicai’
YU Yang-jun;ZHANG Feng-lan;ZHANG De-shuang;ZHAO Xiu-yun;YU Shuan-cang;XU Jia-bing
‘Jingyankuaicai’is a new seedling-edible Chinese cabbage hybrid bred by crossing self-incompatible line 03QX4A23 with 96166.It grows rapidly and can be harvested at 28-30 days after sowing.It has thick,soft,bright and green leaves and white petiole without hair.Its quality is excellent with VC content of 466 mg·kg-1(FW) and soluble sugar content of 8.5 g·kg-1(FW).It is tolerant to heat and wet,and is highly resistant to virus disease(TuMV)and black rot,resistant to downy mildew.Its yield is 60.0-67.5 t·hm-2.It has been extended to Beijing,Tianjin,Hebei,Xinjiang,Yunnan,Guizhou,Sichuan,Hubei,Fujian,Guangdong and Hainan.
2009 Vol. 1 (12): 68-70 [Abstract] ( 1173 ) [HTML 0KB] [ PDF 156KB] ( 909 )
71 A New Mid-early Maturing Cabbage F1 Hybrid —‘Dongnong 611’
WANG Chao;XU Rui-xian;YU Shou-jiang
A new mid-early maturing cabbage F1 hybrid‘Dongnong 611’was released by crossing 3-1028 as female parent and 3-1030 as male parent.Its leafy head is of round shape and very tight density.The external leaf color is grey green.Its leaves are tender and crispy with VC content 452.3 mg·kg-1.It has resistance to TuMV and Black rot.Its single head weights about 2 kg.Its yield is about 75 t·hm-2.It is suitable for production in spring and autumn in Heilongjiang,Jilin,northeast of Inner Mongolia.
2009 Vol. 1 (12): 71-72 [Abstract] ( 1002 ) [HTML 0KB] [ PDF 86KB] ( 645 )
73 A New Eggplant F1 Hybrid with Purple Red Color and Lang Shape —‘Hanhong No.2’
GAO Xu-chun;TAN Tai-ming;XU Chang-cheng;HU Xiao-wen;LIU Ze-pu;GONG Ming-hui;TAN Jie;GUI Wei-xing;CHEN Wei-cai;YU Kai-ran
‘Hanhong No.2’is a new Purple red eggplant F1 hybrid developed by crossing E-2 as female parent with E-9 as male parent.The plant grows vigorously and matures early.It takes about 50 days from flowering to harvesting.The fruit is of long stick shape.It is about 30-35 cm in length and 3.5-4.0 cm in diameter.The average fruit weight is about 150 g.The fruit surface is purple red in color,thin and glossily.The flesh is tender and with a few seeds.It tastes sweet.Its yield is about 52.5 t·hm-2.It is suitable to be cultivated in early spring or late autumn in open field and planstic tunnel in the Yangtze River Valley.
2009 Vol. 1 (12): 73-75 [Abstract] ( 1250 ) [HTML 0KB] [ PDF 100KB] ( 930 )
76 A New Watermelon F1 Hybrid —‘Meiwang’
YUN Wen-jun;ZHENG Jian-li;JIA Hui
A new early maturity watermelon F1 hybrid‘Meiwang’was bred by crossing M16 as female parent and W-2 as male parent.Its fruit development period is about 30 days,and the whole growing period is about 97 days in field planting.But the fruit development period is about 50 days if they were planted in greenhouse.The nodes of fruit set were lower,and its first female flower was sited on the 6th nodes of main vine,the following female flower was sited every 2-3 nodes.Although the growth potential were not blooming,the ability of fruit setting was high and growth uniformity were identical,at the same time it is resistant to low temperature and poor light.Fruit with pellet shape were coved with blank green bands in bottom areas,the color of flesh was bright red and its section was uniform.The soluble solid content in the core area of flesh is 11.6 %,and which is 10.6 % in brim area,the VC content is 84 mg·kg-1.The melon had high quality and good flavor.The single fruit was about 3.5 kg,and the yield were 57 t·hm-2.It can be cultivated in protected or early planted in open field.
2009 Vol. 1 (12): 76-78 [Abstract] ( 1070 ) [HTML 0KB] [ PDF 158KB] ( 749 )
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