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| Detection of Leaf Curl Virus Infected Tomato and Genetic Evolution Analysis |
| TIAN Peijie1，LI Shijun1，ZHANG Yu2，LIU Yong1，2，ZHANG Songbai1，2，ZHANG Deyong1，2* |
| 1Plant Protection Institute of Hunan Agricultural University，Changsha 410120，Hunan，China；2Longping Branch of Graduate College，Hunan University，Hunan Academy of Agricultural Science，Changsha 410125，Hunan，China |
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Abstract From 2017-2019，this experiment collected tomato samples suspected infecting with leaf curl viruses from Gansu，Guangxi，Hainan，Hebei and Yunnan Provinces and conducted analysis and identification on leaf curl viruses species．The results of detection by specific PCR showed that the mean value of positive ratio of tomato samples infected with leaf curl viruses was 25.00%，among them samples from Guangxi was the highest，reaching 50.91%．The results of identity analysis of PCR amplified sequences indicated that infecting 5 provinces’ tomato leaf curl viruses were papaya leaf curl China virus（PaLCuCNV），tomato leaf curl Guangdong virus（ToLCGDV），and tomato leaf curl Guangxi virus（ToLCGXV）．The sequence systematic development phylogenetic analysis revealed that the leaf curl viruses from 5 provinces could be divided into 2 sub-clusters without distinguish regional character and host dependent．Genetic evolution analysis showed that there were 5 recombinant events existed in leaf curl viruses from these 5 provinces，and the highest substitution ratio of 18.73 was existed in C/T．
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Received: 05 November 2020
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