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| Pathogen Identification and Resistant Germplasms for Rootstock Screening of Root-knot Nematode on Solanum melongena in Hainan |
| ZOU Min1，TAO Tao1，YANG Yang1，ZHOU Shanshan1，ZHANG Shicai1，CHEN Guokang2，WANG Yongqing1*，TIAN Shibing1* |
| 1Chongqing Academy of Agricultural Sciences，Chongqing 401329，China；2College of Plant Protection，Southwest University，Chongqing 400716，China |
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Abstract To identify the species of root-knot nematodes（Meloidogyne spp.）of eggplant in Hainan base and select resistant germplasms of eggplant for rootstock．The morphological characteristics and rDNA-ITS molecular biological identifi cation of root-knot nematodes on eggplant were both adopted in this study．Two methods（seedling inoculation and fi eld trials）were used to screen germplasms with high resistance to root-knot nematodes from 58 materials of eggplant for rootstock．The isolation frequency of M. incognita with square dorsal arch was 93.33%，while M. arenaria was 6.67%．NCBI sequences blast showed 99.89% similarity with M. incognita（KP411871.1）by M. incognita primer，and 99.76% similarity with M. arenaria（MW315990.1） by M. arenaria primer．Therefore，M. incognita was the predominant species infecting eggplant in Hainan base．The overall disease severity of the tested germplasms were basically consistent between the two methods．The total disease index of seedling identifi cation was higher than that of fi eld trials．Six eggplant for rootstock germplasms with high resistance to root-knot nematode were screened，which were S. torvum Swartz，S. sisymbriifolium Lam，LJ-7，Ng1-039，Ng1-021，Ng1-003，respectively．
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Received: 21 June 2023
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