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| Identification and Creation of Broad-spectrum Clubroot Resistance Materials |
| LU Shujin1，2，ZHU Mingzhao2，3，ZHANG Na2，FANG Zhiyuan2，YANG Limei2，ZHUANG Mu2，ZHANG Yangyong2，LUY Honghao2，JI Jialei2，YAN Jiping4，WANG Yong2* |
| College of Horticulture，Hunan Agricultural University，Ministry of Education Engineering Research Center of Germplasm
Innovation and New Variety Breeding of Horticultural Crops，Ministry of Education，Key Laboratory of Vegetable Biology of Hunan Province，Changsha 410128，Hunan，China；2Institute of Vegetable and Flower，Chinese Academy of Agricultural Sciences，National Key Laboratory of Vegetable Biological Breeding，Beijing 100081，China；3Beijing Vegetable Research Center，Beijing Academy of Agricultural and Forestry Sciences，State Key Laboratory of Vegetable Bio-breeding，Key Laboratory of Biology and Genetics Improvement of Horticultural Crops （North China），Beijing Key Laboratory of Vegetable Germplasm Improvement，National Engineering Research Center for Vegetables，Beijing 100097，China；4Changli County Agriculturai and rural Bureau，Qinhuangdao 066600，Hebei，China |
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Abstract Clubroot is a soil-borne disease caused by Plasmodiophora brassicae，which seriously threatens the production of cabbage．Due to the complex race differentiation of P. brassicae，there are fewer broad-spectrum resistance materials to clubroot．In this study，the resistance of cytoplasmic sterile clubrootresistant material‘GY306’was identified by spore injection method．The results indicated that‘GY306’was resistant to all P. brassicae from 3 different regions，and the disease grade indexes were immune（Changyang，DI = 0），disease resistance（Wulong，DI = 6.25）and immunity（Guangyuan，DI = 0）respectively．At the
same time，the fertility of‘GY306’was restored by crossing with Ogura CMS fertility recovery material．Finally，three clubroot resistance materials were obtained by identifying and screening the resistance of restored plants．
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Received: 26 March 2023
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