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| Studies on Biological Characteristics of Fruit Rot Pathogen — A New
Muskmelon Disease in Liaoning Province |
| HOU Yue1，LIU Zhi-heng1*，CAO You-wen1，ZHAO Ting-chang2 |
（1College of Plant Protection， Shenyang Agricultual University， Shenyang 110161， Liaoning， China； 2Institute of
Plant Protection， Chinese Academy of Agricultural Sciences， Beijing 100193， China） |
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Abstract The pathogen of muskmelon fruit rot was isolated from the samples of muskmelon from
Liaoning Province．The casual agent was identified as AG-4 HGI of Rhizoctonia solani by the studies
on morphology，pathogenicity，anastomosis groups and rDNA-ITS．The pathogenic biological characteristics
of muskmelon fruit rot were studied in this paper．The result showed that the best medium for
mycelial growth was Muskmelon juice culture medium．The temperature ranged for mycelial growth was
5-40 °C，28 °C was the optimum；the best pH was 7．The effect of light on mycelial growth was not
evident. Mycelial growth was in favor of soluble starch and Peptone．The ideal sclerotia formation was at
25 °C，pH 6-8，Richard culture medium．The suitable carbon source for the formation of sclerotia was
Sorbitol．The beneficial nitrogen source for the formation of sclerotia was KNO3．The sclerotia could
germinate in 10 kinds of nutrition．The proper temperature was 15-40 ℃，and 28 ℃ was the optimum.
Dark condition was conductive to sclerotium germination．The best carbon source and nitrogen source
for sclerotium germination are Malt Sugar and Peptone．Mycelial lethal temperature was 50 ℃，10 min．
The sclerotia lethal temperature was 53℃，10 min.
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Received: 29 October 2012
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