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| Identification and Detection of Pseudomonas syringae pv. tomato in Gansu |
| WEN Zhao-hui1，WANG Jun-ping1，HE Su-qin2* |
（1Gansu Entry-exit Inspection and Quarantine Bureau， Lanzhou 730020， Gansu， China； 2Institute of Plant Protection，
Gansu Academy of Agricultural Sciences， Lanzhou 730070， Gansu， China） |
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Abstract Bacteria strains isolated from diseased tomato fruits were identified as Pseudomonas
syringae pv. tomato based on pathogenicity，morphological characterizations and 16S ribosomal DNA
sequence analysis（ 99% nucleotide identity）．About 530 bp specific fragment of hrpZPst were amplified
from these strains and naturally-infected tomato fruits by specific primers MM5F/MM5R．The results
confirm that this PCR protocol is suitable for specific detecti on of P. syringae pv. tomato in field material.
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Received: 20 November 2012
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