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| Studies on Protein Content，POD and EST Isozyme of Thermo-sensitive
Genic Male-sterile Tomato T-4 |
| GUO Ya-hui1，YU Fen-di2，Gan Gui-yun1，WEI Guan-mu3，WANG Xian-yu1，6*，DU Yongchen
4，Masaharu MASUDA5 |
（1Agronomy College， Guangxi University， Nanning 530004， Guangxi， China； 2Government of Lingnan Town，
Heshan City， Laibin 546500， Guangxi， China； 3Liuzhou City Institute of Agricultural Sciences， Liuzhou 545003，
Guangxi， China； 4Institute of Vegetables and Flowers， Chinese Academy of Agricultural Sciences， Beijing 100081，
China； 5Okayama University, 700-8530， Japan； 6Guilin Comprehensive Experimental Station of National Staple Vegetable
Industrial Technology Systems， Guilin 541004， Guangxi， China） |
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Abstract The experimental materials were thermo-sensitive genic male-sterile tomato（Lycopersicum
esculentum Mill. ）T-4 and the control First．The protein content was determined by spectrophotometry，
adopting Coomassiebrilliant blue G250 as dye．Meanwhile，the activity of POD and
EST isozyme was analyzed with the polyacrylamide gel electrophoresis of perpendicular plate．Results
showed that the protein content of T-4 anther under fertile condition was significantly higher than that
under sterile condition，but was no significant difference with the control First．The band of POD and
EST isozyme in anther of T-4 under sterile condition was more than that in anther of T-4 under fertile
condition，and the control First．There was no significant difference in band of POD and EST isozyme
between T-4 and the control First，when T-4 was under fertile situation.
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Received: 04 November 2012
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