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| Effects of Glycine Concentrations on Nitrogen Metabolism in Plantlet in vitro
of Ginger |
| LIAO Lin-zheng1，ZHU Fei-fei2，LIU Yi-qing1*，TANG Jian-min1，CHEN Ze-xiong1 |
（1Flower Research Institute， Chongqing University of Arts and Sciences， Chongqing Engineering Research Center
for Special Plant Seedlings， Chongqing 402160， China； 2Faculty of Horticulture and Landscape， Southwest University，
Chongqing 400716， China） |
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Abstract In order to determine the effects of organic nitrogen glycine concentration on
physiological characteristics of ginger（ Zingiber officinale Rosc.） plantlet in vitro，this paper
takes plantlet in vitro under aseptic cultivation as experimental material．Five kinds of glycine
concentration level（ 1.0，1.5，2.0，2.5，3.0 mg·L-1） were set up to study the relevant product of
nitrogen metabolism of ginger plantlet in vitro and the activity of nitrogen metabolism key enzyme．
The results showed that the content of chlorophyll and soluble sugars raised with the increase of
glycine concentration，while that of soluble protein was contraire．The activity of GS and GOT were
strengthened with the increase of glycine concentration.
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Received: 23 October 2012
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