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| Genetic Diversity of Chinese Cabbage Analyzed by STR Fluorescence Labeling Method |
| ZHONG Shichao，LI Xiaoxuan，FAN Mingxi，TAN Haoran，CHEN Xiaofeng* |
| Yantai Institute of China Agricultural University，Yantai 264670，Shandong，China |
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Abstract Fingerprints and molecular IDs of cabbage germplasm in Yantai were constructed using STR molecular marker technology with 19 cabbage varieties as test materials．Following the principle of chromosome and amplification band diversity，9 pairs of STR fluorescent primers were screened out，and genetic diversity was analyzed by polyacrylamide gel electrophoresis and multiplexed capillary fluorescence electrophoresis，and STR fingerprints of Chinese cabbage germplasm were constructed，and the results of the fingerprints were coded and assigned，and molecular ID cards were further constructed，and the genetic relationship among varieties was also analyzed by clustering．The results showed that the nine pairs of STR primers screened had good polymorphism，and could effectively distinguish the 19 cabbage varieties
participating in the test．A total of 52 alleles were detected in the nine pairs of primers，and each pair of primers detected 3-8 alleles，with an average of 5.777 8；the Shannon information index（I）varied from 0.714 2 to 1.668 5，with an average of 1.378 7；Nei’s gene diversity index（H）varied from 0.447 4 to 0.789 5，with an average of 0.692 7；polymorphism information content（PIC）varied from 0.368 1 to 0.757 2，withan average of 0.644 2；the amplified STR bands were analyzed and coded，and the molecular identity cards of 19 Chinese cabbage varieties were constructed successfully．The results of cluster analysis showed that when the genetic similarity coefficient（GS）was 0.66，the 19 cabbage varieties were divided into 3 groups，with obvious regional characteristics．
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Received: 20 February 2023
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