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| Molecular Identification of Tomato Sotted Wilt Virus and Detection of Loop-Mediated Isothermal Amplification Technology in Pepper#br# |
| WEI Jianming1， HUANG Xin1， GENG Kun2 ，LI Wenbiao3，FENG Wenzhuo1， LI Yunzhou1* |
| 1Department of Plant Pathology，College of Agiculture，Guizhou University，Guiyang 550025，Guizhou，China；2Vegetable Technology Promotion Station of Guiyang City，Guizhou Province，Guiyang 550081，Guizhou，China；3Bureau of Agriculture and Rural Affairs of Guiyang City，Guiyang 550081，Guizhou，China |
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Abstract This study aimed to identify strains of the tomato spotted wilt virus（TSWV）infecting peppers in Guizhou，and establish rapid detection techniques，specifically TSWV loop-mediated isothermal amplification（LAMP）．We collected Twelve pepper samples suspected of TSWV infection and ten samples of Western flower thrips from major pepper-producing regions in Guizhou Province．These samples were analyzed
using RT-PCR，LAMP detection techniques and hylogenetic tree construction based on TSWV-N genes．Molecular diagnosis and sequencing results confirmed the presence of TSWV in the suspected pepper samples．Additionally，he pathogenic isolates of TSWV in peppers from the Xifeng area of Guizhou exhibited a striking 99% similarity to Guizhou tomato TSWV-N isolates．Building upon these findings，a TSWV rapid detection
system for pepper crops and the vector Thrips occidentalis was successfully established，providing strong support for the monitoring and control of pepper virus diseases in Guizhou．
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Received: 24 June 2022
Published: 05 December 2022
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