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| Phenotype Analysis and Primary Mapping of A Short-internode Mutant si302 in Watermelon |
| MA Cai’e1，2☆，XIAO Yanhong1，2☆，LIU Tangjing3☆，GONG Guoyi2，ZHANG Jie2，ZHANG Haiying2* |
| 1College of Plant Science and Technology，Beijing University of Agriculture，Beijing 102206，China；2Vegetable Research Institute，Beijing Academy of Agriculture and Forestry Sciences，Beijing 100097，China；3Horticultural Research Institute，Guangxi Academy of Agricultural Sciences，Nanning 530007，Guangxi，China |
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Abstract We obtained a short internode mutant si302 by ethyl methane sulfonate（EMS）mutagenesis of watermelon inbred line 302．In this study，we collected the agronomic traits of six-basic generations（P1，P2，F1，F2，BC1P1 and BC1P2）from the cross between 302 and si302．Genetic analysis and bulked segregant analysis sequencing（BSA-seq）were used to located the candidate genes for the short internode trait in si302．Results showed that plant height，stem length and stem internodes of si302 were decreased than 302．But si302 had compacted plant shape and normal fruit traits paraffin section showed that the si302 internode cell length is shorter than 302．Genetic analysis showed that the si302 mutant is inherited in a 3∶1 ratio（χ2 = 0.16），the short internode mutant phenotype of si302 was controlled by one recessive gene．With the strategy of BSA-seq，the short internode mutant was primary mapped into 36.0-36.7 Mb region on chromosome 1．
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Received: 05 May 2023
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