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| Applying Sucrose Promotes Cucumber Lateral Branch Outgrowth |
| ZHANG Zeqin，ZHOU Zhaoyang*，ZHANG Xiaolan |
| State Key Laboratories of Agrobiotechnology，Beijing Key Laboratory of Growth and Development Regulation for Protected Vegetable Crops，MOE Joint Laboratory for International Cooperation in Crop Molecular Breeding，College of Horticulture，China Agricultural University，Beijing 100193，China |
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Abstract Shoot branching is one of the major agronomic characters，regulated by many factors．Saccharide plays important function in development of plant lateral branches as a signal substance．But its function in cucumber lateral branch development was rarely reported．Taking cucumber line‘461’as material，this experiment conducted spraying treatment by sucrose solution of different concentrations to promote the lateral branch elongation．Of all concentrations，40 mmol · L-1 sucrose solution had the most obvious promotion effect．Through transcriptome sequencing RNA-seq，all together 568 gene differential expressions were detected after sucrose treatment．GO analysis discovered differentially expressed genes were mainly gathering during the biological process，including biological rhythm，signal transduction，and transcription factor activity，etc.．The result of real-time PCR showed that BRC1 gene related to the lateral branch outgrowth was down-regulated．Above results indicated that sucrose regulated the length of cucumber lateral branch by regulating expression of some genes related to lateral branch development．
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Received: 17 February 2022
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