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| Effects of Seed Soaking with Spermidine on Tomato Seed Germination and Seedling Tolerance to High Temperature |
| WANG Yu，LIU Guo-zhou，CAI Li-cong，GUO Shi-rong* |
| College of Horticulture，Nanjing Agricultural University，Nanjing 210095，Jiangsu，China |
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Abstract Taking tomato（Solanum lycopersicum L. ）variety ‘Hezuo 903’as material，and treating the seeds with 0，0.1，0.2，0.4 mmol · L-1 spermidine（Spd） for 10 hours，this paper studied the effect of different concentrations of Spd on seed germination，seedling growth and high temperature tolerance．The results showed that the seed soaking treatment with different Spd concentrations could significantly increase the germination index and vigour index of tomato seeds．The underground root dry weight of tomato seedlings and strong seedling index could also be remarkably increased．Among them，the germination rate and vigour index under 0.2 mmol · L-1 Spd treatment were the fastest and highest．Spd treatment could reduce the relative electrolyte permeability of tomato leaves under high temperature stress，contents of malondialdehyde（MDA）and H2O2．The relative electrolyte permeability，MDA and H2O2 contents under 0.2 mmol · L-1 Spd treatment were the lowest，while the activities of antioxidant enzyme（SOD，APX，DHAR）were increased and reached the peak value when treated with 0.2 mmol · L-1．To sum up，seed soaking with 0.2 mmol · L-1 Spd treatment could effectively promote tomato seed germination and seedling growth and improve tomato seedlings tolerance to high temperature．
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Received: 02 June 2019
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