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| Applicability Evaluation and Application of Linked Markers for Restorer-offertility in Pepper |
| MI Zhi-bo，ZHANG Jian-ying，WANG Ping-yong，LIU Chen，SHEN Huo-lin* |
| College of Agronomy and Biotechnology，China Agricultural University，Beijing Key Laboratory of Growth and Development Regulation for Protected Vegetable Crops，Beijing 100193，China |
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Abstract In order to accurately and efficiently use molecular marker assisted selection technique in selecting Restorer-of-fertility gene in pepper（Capsicum annuum L.） lines，this experiment carried out applicability test on the 11 pairs of reported Restorer-of-fertility gene linked markers with 36 pepper inbred line，whose genotype（RfRf or rfrf） has been known. The results indicated that dominant markers CRF3S1S and CRF-SCAR had the broadest applicability，and their accuracy rate were 91.67% and 88.89%，respectively. CaRf-FL-M2 had the broadest applicability among the 6 pairs of co-dominant markers，and its accuracy rate was 80.56%. In breeding practice，CRF3S1S or CRF-SCAR markers could be used to preliminarily identify pepper materials. Then one more identification could be conducted on pepper material containing Rf gene by CaRf-FL-M2 marker.
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Received: 06 March 2014
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