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| Preliminary Study on Cold-induced Transcription Factor CBF3 Transformed
in Eggplant |
| MENG Ping-hong1，WAN Fa-xiang2，WANG Yong-qing3，WEN Lin-hong1，GUO Jing-tao1，SU
Cheng-gang2* |
1Institute of Horticulture， Guizhou Academy of Agricultural Sciences， Guiyang 550006， Guizhou，C hina； 2Key
Laboratory of Horticulture Science for Southern Mountainous Regions， Ministry of Education， College of Horticulture
and Landscape Architecture， Southwest University， Chongqing 400715， China； 3Institute of Vegetables and
Flowers， Chongqing Academy of Agricultural Sciences， Chongqing 400700， China |
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Abstract We successfully constructed cold-induced transcription factor CBF3（CRT binding
factor），which was transferred to eggplant‘ Sanyueqie’（ Solanum melongena L.）by agrobacteriummediated
transformation. And we obtained 2 transgenic eggplant plants containing cold-tolerant
gene CBF3，through Polymerase Chain Reaction（PCR）detection genomic DNA，and kanamycin
solution daubing on the leaves of generation plants in the field confirmed that the transgenic plants of
eggplant were positive．Thus，we successfully established regeneration and transformation systems
in eggplant.
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Received: 04 February 2013
Published: 06 April 2013
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