IN2-2：：GUS transgenic mustard was treated with different concentrations（50、100、200 mg·L-1）of acetanilide and 2-chlorobenzenesulfonamide，the herbicide safer analogous chemicals．The results showed that IN2-2 promoter expressed in mustard roots，leaves，flower calyx，petal，stamen and pollen after treated with acetanilide or 2-chlorobenzenesulfonamide，but no GUS expression activity was observed in ovule．100 mg·L-1 acetanilide or 50 mg·L-1 2-chlorobenzenesulfonamide solution were suitable for the expression of IN2-2 promoter in mustard，and the acetanilide induce the IN2-2 promoter expression more quickly than 2-chlorobenzenesulfonamide in all tissues and organs．In addition，high concentration 2-chlorobenzenesulfonamide treatment was detrimental to mustard germination and growth，and would obviously inhibit the expression activity of IN2-2 promoter．4 ℃ temperature stress induced IN2-2 promoter expression in seedling leaf blades．This promoter expression was slightly induced by 150 mmol·L-1 NaCl，but not by heavy metalions Gu2+．