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| Isolation， Indentification and Evaluation for Nematicidal Activity of Actinomycetes Against Plant Nematode Meloidogyne incognita |
| CHEN Jing-Sheng, ZHU Feng, LU Xu-Peng, LIU Da-Wei, LIU De-Fu, DUAN Yu-Xi, CHEN Li-Jie |
| 1Daqing Branches of Heilongjiang Academy of Agricultural Sciences， Daqing 163316， Heilongjiang， China； 2Nematology Institute of Northern China， College of Plant Protection， Shenyang Agricultural University， Shenyang 110866，Liaoning， China； 3Plant Protection Station ofLiaoning Province， Shenyang 110034， Liaoning， China |
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Abstract A strain H-2 was isolated by diluted solution method from soybean continuous cropping soil sample，its morphological，physiological and biochemical characteristics were identified and 16S rDNA was analyzed. The result showed that it was Streptomyces parvus. Fermentation filtrates of Streptomyces parvus at different concentrations were evaluated for nematicidal activity against Meloidogyne incognita and Rhabditis sp. in laboratory. Fermentation filtrates of the strains could suppress the egg hatching and 2nd-instar larvae of Meloidogyne incognita. Culture filtrates of Strain H-2 showed a weak level of nematicidal activity of free-living Rhabditis sp. The corrected mortality rate of Meloidogyne incognita egg hatching and 2nd-instar larvae reached 74.35% and
78.46%，respectively by， strain H-2 at 5 diluted solution.
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Received: 11 July 2014
Published: 19 January 2015
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