|
|
|
| Screening of Methods to Extract Total RNA from Micro-spore of Chinese Cabbage Suitable for Transcriptome Sequencing |
| LIN Yan1，2，LI Fei1，ZHANG Shu-jiang1，ZHANG Hui1，ZHANG Shi-fan1，SUN Ri-fei1*，ZHANG Zhen-xian2 |
（1Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China；2College of
Agronomy and Biotechnology，China Agricultural University，Beijing 100193，China） |
|
|
|
|
Abstract In order to find out micro-spore extracting method suitable for transcriptome sequencing，this study took Chinese cabbage‘Jihong 82’as experimental material and micro-spore induced with heat shock as research object．This study compared and analyzed the RNA extraction effects by 3 different methods：TRIzol method，improved CTAB method and ultrapure kit method．The results showed that RNA concentrations extracted by TRIzol method and improved CTAB method were higher，but the RNA integrity was lower．Quality of RNA extracted by ultrapure kit method was the highest，and the RNA inegrity was also good．Therefore，RNA obtained by this method was suitable for satisfying the requirement of transcriptome sequencing．
|
|
Received: 21 March 2014
Published: 16 May 2014
|
|
|
|
|
|
|
