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| Genetic Diversity of BrAOP2 and Analysis of Its Correlation with Glucosinolates Content in Chinese Cabbage |
| LIU Zhi-yuan，ZHANG Ji-fang，WU Jian，LIANG Jian-li，WANG Xiao-wu * |
| （Institute of Vegetables and Flowers， Chinese Academy of Agricultural Sciences， Beijing 100081， China） |
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Abstract In this study，full length cDNA sequence of 3 BrAOP2 genes（BrAOP2.1，BrAOP2.2，
BrAOP2.3）was cloned from Chinese cabbage〔Brassica campestris L. ssp. pekinensis（Lour） Olsson〕
‘Chiifu’．Sequence alignment showed that BrAOP2.1 shares 78% identity with BrAOP2.2 and 74% with
BrAOP2.3，while BrAOP2.2 and BrAOP2.3 share 81% identity．Glucosinolates profile and contents
were evaluated for 32 Chinese cabbage accessions． 7 glucosinolates were detected by HPLC. Glucobrassicanapin（
GBN） and Progoitrin（ PRO） were the most dominant glucosinolates with the highest ratio
around 23.99% and 23.16% of the total glucosinolates，respectively．Among the 32 accessions，13
SNPs were identified for BrAOP2．The correlation analysis between identified BrAOP2 SNPs and glucosinolates
content revealed that A1051G site of BrAOP2.1 and A560G，C753T，A790G and T927C
of BrAOP2.2 had significant correlation with the PRO content，while the G825A site of BrAOP2.2 had significant correlation with 4-hydroxyglucobrassicin（4OH）conten t.
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Received: 31 May 2013
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