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| Optimization of Conditions for Identifying Anthracnose in Phaseolus vulgaris L. Seedling Stage and Resistance Assessment of Its Germplasm Resources |
| HAN Qi-hou，GU Yu，YU Hai-long，XIA Jun-feng，LIU Hui-jing，YUE Xin， LANG Lang |
| Tianjin Kernel Vegetable Research Institute， Tianjin 300384， China |
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Abstract The identification conditions including sporulation medium，bacterial concentration，culture time and inoculation position for anthracnose resistance inoculation during bean（Phaseolus vulgaris L.）seedling stage were optimized．The suitable medium was bean pod medium with rapidly spore growth and strong pathogenicity．The best concentration of vaccination was about 2.0×106 spores per mL，with abrupt onset and good reproducibility．After 20 days’ culture，its pathogenicity was the strongest with best effect of inoculation．The best inoculation position was on the back of leave blades．It might save time．All 23 bean materials were identified for their resistance to anthracnose．Among them 4 materials were mid-resistant，1 was high resistant and most of the resistance resources were of rape bean type.
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Received: 20 November 2012
Published: 15 January 2013
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