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| Studies on Tomato Marker-free Transformation System |
| ZOU Fang-ling，GUO Yan-mei，DU Yong-chen，ZHU Guang-tao，GAO Jian-c hang，HU Hong* |
| Institute of Vegetables and Flowers， Chinese Academy of Agricultural Sciences， Beijing 100081， China |
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Abstract In this paper，the aimed gene GGPS was cut from the binary plant expression vector pBI121-GGPS and ligated to the marker-free vector pBINMF-GUS，so a new marker-free vector was formed，in which T-DNA segment only harboured GGPS gene and a GUS gene. This vector was successfully transferred into tomato（Lycopersicum esculentum Mill. ） cultivar‘ Zhongshu No. 6’ via Agrobacterium-mediated transformation used strains EHA105 and C58. The results showed that MS+1.0 mg·L-1 ZT+0.5 mg·L-1 IAA was the best shooting medium for‘ Zhongshu No. 6’. The transformation rate of strain EHA105 was 3.6%，which was better than the rate of strain C58. The results of PCR analysis indicated that GGPS gene was integrated into the genome of‘Zhongshu No. 6’. RT-PCR detection indicated that GGPS gene was expressed at RNA level.
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Received: 16 January 2013
Published: 15 March 2013
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