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| Studies on Potato Transformation of BDN1 Gene Mediated by Agrobacterium
tumefaciens |
| PAN Ying-xue1，ZHANG Li-li1*，SHI Ying1，LU Cui-hua1，LIN Zhong-ping2 |
1College of Agronomy， Northeast Agricultural University， Harbin 150030， Heilongjiang, China； 2College of Life
Science， Peking University， Beijing 100871， China） |
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Abstract The BDN1 gene in coding area from drought-tolerant Boea crassifolia Hemsl was
transferred into potato（Solanum tuberosum L.） cultivars‘ Dongnong 303’ through agro-bacteriummediated
transformation. The factors influencing its genetic transformation were studied. Thirty-five
transgenic plants with kanamycin resistance were obtained. Specific primers were used to amplify the full
length of the BDN1 gene from total genomic DNA of the transgenic plants. Among them, 27 plants were
positive by PCR test and 4 plants were positive by Southern-blot test. The result of semi-quantitative
RT-PCR test showed that BDN1 gene was inserted into the genome of the transgenic plants and can be
transcribed normally. The physiological index showed the transgenic potato plants were significantly
tolerant to drought stress.
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Received: 28 November 2012
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