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Real-time Quantitative PCR Technology for Detecting Vegetable Bacterial
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CHEN Lu,XIE Xue-wen,SHI Yan-xia,LI Bao-ju * |
Insititute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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Abstract Real-time quantitative polymerase chain reaction( PCR) method offers new train of thoughts to study phytopathogenic pathogens and to control plant diseases timely.This paper expounds the theory of real-time quantitative PCR,its specificity,sensitivity,application in detecting vegetable
bacterial diseases recently and prospects for development of real-time PCR.This paper has provided theoretical foundation and technical support for studying vegetable bacterial pathogens and diseases control.
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Received: 18 January 2013
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