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| Analysis of Differentially Expressed Genes in Chinese Cabbage Hybrid and Its Parents |
| LIN Jian-li1，2，ZHANG Yi-hui2，LI Li-bin2，WANG Feng-de2，ZHU Zheng-ge3，GAO Jian-wei2* |
| 1College of Huihua，Hebei Normal University，Shijiazhuang 050091，Hebei，China|2Institute of Vegetables，Shandong Academy of Agricultural Sciences，Shandong Branch of National Vegetable Improvement Center，Shandong Key Laboratory of Green Vegetable Biology，Jinan 250100，Shandong，China|3College of Life Sciences，Hebei Normal University，Shijiazhuang 050016，Hebei，China |
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Abstract In this study，the differential gene expression among Chinese cabbage〔Brassica campestris L. ssp. pekinensis（Lour）Olsson〕hybrid‘Baiyin No.2’and its parents was analyzed by cDNA-AFLP．The results showed that 29 differentially expressed fragments were characterized in 3 different stages，of which 5 differentially expressed fragments are in the seeding stage，14 differentially expressed fragments in the rosette stage，10 differentially expressed fragments in the heading stage．19 difference fragments expressed only in the hybrid，3 difference fragments expressed only in parents，2 difference fragments expressed only in female parent and 5 difference fragments expressed only in male parent．The comparative analysis of 29 differentially expressed fragment sequences with Chinese cabbage genome sequence result discovered that 29 bands had their locations in Chinese cabbage genome database website（http://brassicadb.org/brad/），of which 17 differentially expressed fragments were corresponding genes coding proteins，respectively．These 17 differentially expressed genes participated in the following biological process：metabolism and cellular transport，etc．Of these，2 genes included the AP2/EREBP domain and PPR domain，respectively．These 2 domains play important roles in controlling plant growth and development．
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Received: 12 December 2011
Published: 29 January 2012
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