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| Studies on Fertility of Thermo-sensitive Genic Male-sterile Tomato，Lycopersicum esculentum Mill.—T4 |
| WANG Xian-yu1，6，YU Fen-di1，LIANG Cong-yao1，LI Liang-shao2，HUANG Wei-na1，DU Yong-cheng3，LIU Zheng-guo1，ZENG Dong4，PAN Li-fan5 |
| 1Agronomy College，Guangxi University，Nanning 530004，Guangxi，China|2Rong’an County Cash Crop Extension Station，Liuzhou 545400，Guangxi，China|3Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China|4Government of Guangxi Zhuang Autonomous Region，Nanning 530012，Guangxi，China|5Qingxiu District Bureau of Water Conservancy，Agriculture and Forestry, Nanning 530213，Guangxi，China|6Guilin Comprehensive Experimental Station of National Staple Vegetable Industrial Technology Systems，Guilin 541004，Guangxi，China |
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Abstract Taking thermo-sensitive genic male-sterile tomato Lycopersicum esculentum Mill．T4 and the control First as experimental materials，this paper investigated pollen germination rate，self-breeding rate and fruit seed number，analyzed the protein changes in leaf and anther by Tricine-SDS-PAGE electrophoresis to comprehend fertile conversion of T4．The paper also analyzed the pollen germination rate，self-breeding rate and fruit seed number，protein differences in leaf and anther under fertility and sterility conditions．The results showed that pollen germination rate，self-breeding rate and fruit seed number of T4 were the highest with 28 ℃/18 ℃（day/night）treatment．There was no significant difference compared with the control First．It indicated that T4 had restored partial fertility．But，with 28 ℃/24 ℃ and 28 ℃/12 ℃ treatments the pollen germination rate，self-breeding rate and fruit seed number of T4 had significant differences with the control First，and T4 expressed as sterile line．There were significant differences in leaf protein and anther protein of T4 under different temperature treatments．Under 28 ℃/24 ℃ and 28 ℃/12 ℃ treatments，leaf protein of T4 was significantly different with the control First．But，there was no significant difference between T4 and the control under 28 ℃/18 ℃treatment.
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Received: 18 November 2010
Published: 10 January 2011
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