1College of Horticulture，Xinjiang Agricultural University，Xinjiang Characteristic Horticultural Crop Germplasm Resources and Efficient Production Laboratory，Urumqi 830052，Xinjiang，China；2Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，State Key Laboratory of Vegetable Biobreeding，Beijing 100081，China；3Ganzhou
Agricultural School，Ganzhou 341000，Jiangxi，China
Abstract：Aiming at the lack of stable and highly polymorphic SNP markers of peppers，KASP markers were designed at a spacing of 20 cM according to the correspondence between the high-density genetic map and the physical map of peppers，and a total of 234 pairs of KASP primers were designed．F2 isolated population screening was used to obtain 159 pairs of primers with polymorphism，and the success rate of labeling design was 67.9%．Using a group of 207 peppers from 14 introduction sites，the polymorphism and stability of primers were verified for high-throughput system transformation and labeling．The polymorphism of 141 selected markers was detected by high-throughput genotyping system，of which 130 KASP markers were successfully typed，with a success rate of 92%，and the number of markers with polymorphism in pepper materials varied from 24-129，and the corresponding polymorphism site percentage range was 18.46%-99.23%．A set of KASP markers containing 130 pepper KASP markers was developed and evenly distributed on the 12 chromosomes of peppers，and through the typing of 14 domestic pepper varieties from the source of introduction，it was proved that this set of marker polymorphisms is highly polymorphic and universal，and is suitable for high-throughput genotyping of pepper varieties.
